Meningeal cells surrounding the brain participate actively in the normal development of the central nervous system. For example, they play important roles in both stabilizing the extracellular matrix of the pial surface and by organizing the radial glial scaffold and the lamination of the cerebellar cortex [1]. Selective pharmacological destruction of the meningeal cells during a critical ontogenetic period leads to specific malformation of both the cerbella cortex and dentate gyrus [1]. Grafts of meningeal cells, which derived form meninges overlying the cerebral cortex, in adult rat spinal cord lesion promote axonal regrowth [2]. In vitro study show that meningeal cell chemotactically orient the migration of immature neurons but not glial cells [3].
RMC from ScienCell Research Laboratories are isolated from rat leptomeningi. RMC are cryopreserved at passage one cultures and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. RMC are characterized by immunofluorescent method with antibodies to fibronectin and negative to GFAP, alpha-smooth muscle actin and Thy 1.1. RMC are negative for mycoplasma, bacteria, yeast and fungi. RMC are guaranteed to further expand for 15 population doublings in the conditions specified by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Meningeal Cell Medium (MCM, Cat. No. 1401) for the culturing of RMC in vitro.
Product Use
RMC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
Storage
Transfer directly and immediately cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Shipping
Dry ice.
Reference
[1] Hartmann, D., Sievers, J. Pehlemann, F. W. and Berry, M. (1992) Destruction of meningeal cells over the medial cerebral hemisphere of newborn hamster prevents the formation of the infrapyramidal blade of the dentate gyrus. J. Comparative Neurol. 320:33-61.
[2] Franzen, R., Martin, D., Daloze, A., Moonen, G. and Schoenen, J. (1999) Grafts of meningeal fibroblasts in adult rat spinal cord lesion promote axonal regrowth. Neuroreport 10:1551-1556.
[3] Hartmann, D., Schulze, M. and Sievers, J. (1998) Meningeal cells stimulate and direct the migration of cerebellar external granule cells in vitro. J. Neurocytol. 27:395-409.
