The tissue of the central nervous system is made up of two classes of cells that may be broadly
categorized as neurons and glia. Neurons are anatomic, functional, and trophic units of the brain
[1]. Despite great variability in size and shape, all neurons share common morphologic features,
which are those of the key elements of a highly complex communication network. The neurons
are the dynamically polarized cells that serve as the major signaling unit of the nervous system.
The human brain is known to contain about 1 x 1011 neurons, each being able to contact at least
10,000 other neurons [2].
RN-sn from ScienCell Research Laboratories were isolated from E14 rat brain cortical. RN-sn
were cryopreserved at primary culture and delivered frozen. Each vial contains >1 x 106 cells in
1 ml volume. RN-sn are characterized by immunofluorescent method with antibodies to
neurafilament, MAP2, and beta-tubulin III. RN-sn are negative for mycoplasma, bacteria, yeast
and fungi. RN-c are guaranteed to further culture in the conditions provided by ScienCell
Research Laboratories.
Recommended Medium
It is recommended to use neuronal medium (NM, Cat. No. 1521) for the culturing of RN-sn in
vitro.
Product Use
RN-sn are for research use only. They are not approved for human or animal use, or for
application in in vitro diagnostic procedures.
Storage
Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep
the cells in liquid nitrogen until cell culture is needed for experiments.
Shipping
Dry ice.
Reference
[1] Parent, A. (1996) Neurons in Carpenter's Human Neuroanatomy. 9th ed., pp131-198, Williams & Wilkins,
Quebec, Canada.
[2] Alberts, B., Bray, D., Lewis, J., Raff, M., Roberts, M., Watson, J. D. (1989) Molecular biology of the cell. 2nd.
ed., New York: Garland.
Click the picture for large image
Fig 1. Immunofluorescent staining of passage zero RN-sn with anti-tubulin monoclonal antibody(green) and DAPI(blue), 400X.