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How can we help you today?
Need help with product selection and ordering?
Email us at sales@sciencellonline.com
Need help with scientific inquiries, technical assistance, product applications, and use?
Email us at techsupport@sciencellonline.com
Need help with issues concerning this website?
Email us at webmaster@sciencellonline.com
Have any marketing related inquiries?
Email us at marketing@sciencellonline.com
Need help ordering from abroad?
As the largest cell provider, we are available worldwide.
Please visit our Distributors page to select the company nearest to your location.
We are located at 1610 Faraday Ave, Carlsbad, CA 92008
Call us at (877) 602-8549 or (760) 602-8549
Fax us at (760) 602-8575
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Age-related macular degeneration (AMD) is characterized in its early stages by the presence of extracellular deposits, known as drusen, that accumulate between the basal surface of the retinal pigmented epithelium and Bruch’s membrane, an extracellular matrix complex that separates the neural retina from the capillary network in the choroid [1]. Several studies have shown that drusen contains a variety of protein and lipid components [2]. Although liver is the primary biosynthetic site for most of these molecules, retinal pigment epithelial (RPE) cells locally synthesize a number of drusen components [2]. The respective contributions of RPE-derived and plasma-derived molecules to the biogenesis of drusen, and the relevant molecular interactions leading to drusen depositions, however, have not been fully identified. One of the major limitations is that RPE cells, once isolated from the eye, tend to dedifferentiate into myofibroblasts in conventional 2D cell culture. Recent studies have shown that 3D retinal pigment epithelial cell spheroids form and maintain a well-differentiated epithelium in 3D cell culture [3]. ScienCell develops an all-inclusive 3D human retinal pigment epithelial spheroid formation kit (3D-HRPEpiSF). 3D RPE spheroids exhibit the differentiated epithelial cell marker cytokeratin-18 and deposit apolipoprotein ApoE, a prominent drusen constituent. The 3D RPE spheroid model is an ideal way to model drusen in vitro and study the pathogenesis of related diseases, such as AMD.
Kit Components:
3D Cell Culture Components | ||||
---|---|---|---|---|
Cat # | # of vials | Product Name | Quantity | Storage |
6540 | 1 | Human Retinal Pigment Epithelial Cells (HRPEpiC) | 5 × 10^5 cells | Liquid nitrogen |
3D-4101 | 1 | 3D-Epithelial Spheroid Medium (3D-EpiSpM) | 200 mL | 2-8 °C |
3D-4152 | 1 | 3D-Epithelial Spheroid Supplement (3D-EpiSpS) | 2 mL | -20 °C |
0004 | 1 | Fetal Bovine Serum (FBS) | 4 mL | -20 °C |
0583 | 1 | Penicillin/Streptomycin Solution (P/S) | 2 mL | -20 °C |
0343 or 0353 or 0383 | 1 | Ultra-Low Binding Culture Plates (24-, 48-, or 96- well plate) | 1 plate | RT |
2D Cell Culture Components | ||||
Cat # | # of vials | Product Name | Quantity | Storage |
4101 | 1 | Epithelial Cell Medium (EpiCM) | 500 mL | 2-8 °C |
4152 | 1 | Epithelial Cell Growth Supplement (EpiCGS) | 5 mL | -20 °C |
0010 | 1 | Fetal Bovine Serum (FBS) | 10 mL | -20 °C |
0503 | 1 | Penicillin/streptomycin Solution (P/S) | 5 mL | -20 °C |
Additional Recommended Materials (Not Included):
Cat # | Product Name |
0113 | Trypsin Neutralization Solution |
0183 | 0.05% Trypsin/EDTA (T/E) |
0303 | Dulbecco’s Phosphate-Buffered Saline (DPBS) |
0413 | Poly-L-Lysine (10 mg/mL) |
Catalog No. | 3D-6540 |
---|---|
Country of Manufacture | United States |
Product Code | 3D-HRPEpiSF |
Size/Quantity | 1 Kit |
Product Use | This product is for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures. |
Storage | Refer to product sheet. |
Shipping Info | 6540, 3D-4152, 0004, 0583, 4152, 0010, and 0503 are shipped on dry ice. 3D-4101, 4101, and (0343 or 0353 or 0383) are shipped at room temperature. |
No Publication available at this time
CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
---|---|---|---|---|---|
SP3D-6540 | SP3D-HRPEpiS | Ready-To-Use 3D Human Retinal Pigment Epithelial Spheroids |
$1,236.00
|
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