Colorimetric Histone Acetyltransferase Activity Assay (HAT) Catslog #8668 100 Tests in 96-well plate

Histone acetyltransferases (HATs) catalyze the acetylation of histone proteins by transferring an acetyl group from cofactor acetyl-CoA, and consequently regulate gene expression. The regulation is critical for cellular processes such as cell differentiation and proliferation. Our Colorimetric Histone Acetyltransferase (HAT) Activity Assay Kit offers rapid and sensitive detection of HAT activity or HAT inhibition in mammalian cells/tissue samples. Briefly, acetylation of a peptide substrate by HATs releases the free form of CoA, which can serve as a coenzyme of a NADH generating enzyme to produce NADH. The stoichiometric NADH production can be quantified by spectrophotometric analysis at 440 nm upon reacting with water soluble tetrazolium-1 (WST-1), a soluble tetrazolium dye. The HAT assay does not require radioactivity or chromatography and can be applied for kinetic studies. HATs' counteracting enzymes, histone deacetylases (HDACs), do not interfere with the assay, consequently crude nuclear extract or cell lysate samples can be used.

Catalog No.	8668
Country of Manufacture	United States
Product Code	HAT
Size/Quantity	100 Tests in 96-well plate
Product Use	This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Storage	Upon receipt, components #8668a, 8668c, 8668e, 8668f and8668h should be stored at -20°C, #8668b, 8668d and 8668g should be stored at -80°C. Protectfrom light. Repeated freeze/thaw cycles should be avoided. Aliquot if necessary. If storedproperl
Shipping Info	Dry ice.