Custom SNP Genotyping Kits
ScienCell's GeneQuery™ custom SNP genotyping kits by Melt Curve Analysis allow you to identify the sample genotype (A/A, A/B or B/B) at a target genomic location in 90 minutes.
For each sample, set up 2 qPCR reactions, one with primer set A (psA), and one with primer set B (psB). Following qPCR with melt curve analysis, the melt temperature (Tm) of the qPCR product is compared to a predetermined threshold temperature (Tthreshold). The interpretation of results is shown in Table 1.
| qPCR with Primer set A | qPCR with Primer set B | Interpretation |
|---|---|---|
| Tm,psA > TThreshold, A | Tm,psB < TThreshold, B | Genotype A/A |
| Tm,psA < TThreshold, A | Tm,psB > TThreshold, B | Genotype B/B |
| Tm,psA > TThreshold, A | Tm,psB > TThreshold, B | Genotype A/B |
$890.00
All custom SNP genotyping kits will be wet-lab verified. The normal turnaround time is 5 to 15 business days. Please contact us at 877-602-8549 or info@sciencellonline.com
| Custom SNP primer set A, lyophilized | 250 reactions |
| Custom SNP primer set B, lyophilized | 250 reactions |
| Nuclease-free H2O | 10 mL |
| 2x SYBR® Green qPCR master mix | 5 mL |
