Endothelial Cell Medium

Endothelial Cell Medium (ECM), when used with Endothelial Cell Growth Supplement (ECGS, Cat #1052) and 25 ml of fetal bovine serum (FBS) is a complete medium designed for optimal growth of normal endothelial cells in vitro. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (5%). The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide an optimally balanced nutritional environment that selectively supports the growth of normal endothelial cells in vitro.

Catalog No.	1001
Country of Manufacture	United States
Product Code	ECM
Size/Quantity	500 ml
Product Use	This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Storage	Store the basal medium at 4°C, the ECGS, the FBS and the P/S solution at -20°C. Protect from light.
Shipping Info	Basal medium: room temperature. Supplements: dry ice.

Less Number of Supplements Review by Barbara

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This Media is Low Serum suitable to culture endothelial cells from large blood vessels. the best thing is the less number of supplement which helps us in less human error. Thank you sciencell, keep up the good work.

(Posted on 4/13/2017)

The medium is very good for the HAEC and HBMVEC cells Review by Grace

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The Endothelial Cell Medium is very good for the HAEC and HBMVEC cells. Cells growing very well. (Posted on 4/1/2017)

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1.) Zhu, D.D., Tang, R.N., Lv, L.L., Wen, Y., Liu, H., Zhang, X.L., Ma, K.L. & Liu, B.C. (2016) Interleukin-1Beta mediates high glucose induced phenotypic transition in human aortic endothelial cells Cardiovasc Diabetol. 15

2.) Zhou, Y., Zhang, H., Zheng, B., Ye, L., Zhu, S., Johnson, N.R., Wang, Z., Wei, X., Chen, D., Cao, G., Fu, X., Li, X., Xu, H.Z. & Xiao, J. (2016) Retinoic Acid Induced-Autophagic Flux Inhibits ER-Stress Dependent Apoptosis and Prevents Disruption of Blood-Spinal Cord Barrier after Spinal Cord Injury Int J Biol Sci. 12

3.) Zhou, C., Zhang, Y., Dai, J., Zhou, M., Liu, M., Wang, Y., Chen, X.Z., Tang, J., Abu, N., Akhtar, M.N., Yeap, S.K., Lim, K.L., Ho, W.Y., Abdullah, M.P., Ho, C.L., Omar, A.R., Ismail, J. & Alitheen, N.B. (2016) Pygo2 functions as a prognostic factor for glioma due to its up-regulation of H3K4me3 and promotion of MLL1/MLL2 complex recruitment Flavokawain B induced cytotoxicity in two breast cancer cell lines, MCF-7 and MDA-MB231 and inhibited the metastatic potential of MDA-MB231 via the regulation of several tyrosine kinases In vitro Sci Rep. 6

4.) Zhao, Y., Qiao, X., Wang, L., Tan, T.K., Zhao, H., Zhang, Y., Zhang, J., Rao, P., Cao, Q., Wang, Y., Wang, Y., Wang, Y.M., Lee, V.W.S., Alexander, S.I., Harris, D.C.H. & Zheng, G. (2016) Matrix metalloproteinase 9 induces endothelial-mesenchymal transition via Notch activation in human kidney glomerular endothelial cells BMC Cell Biol. 17

5.) Zhang, Z., Zhang, H., Peng, T., Li, D. & Xu, J. (2016) Melittin suppresses cathepsin S-induced invasion and angiogenesis via blocking of the VEGF-A/VEGFR-2/MEK1/ERK1/2 pathway in human hepatocellular carcinoma Oncol Lett. 11

6.) Yan, X., Cao, J., Liang, L., Wang, L., Gao, F., Yang, Z., Duan, J., Chang, T., Deng, S., Liu, Y., Dou, G., Zhang, J., Zheng, Q., Zhang, P. & Han, H. (2016) miR-342-5p Is a Notch Downstream Molecule and Regulates Multiple Angiogenic Pathways Including Notch, Vascular Endothelial Growth Factor and Transforming Growth Factor Beta Signaling J Am Heart Assoc. 5

7.) Xie, Y., Zhong, Y., Gao, T., Zhang, X., Li, L., Ruan, H. & Li, D. (2016) Human lymphatic endothelial cells contribute to epithelial ovarian carcinoma metastasis by promoting lymphangiogenesis and tumour cell invasion Exp Ther Med. 11

8.) Xie, F., Li, Z.P., Wang, H.W., Fei, X., Jiao, Z.Y., Tang, W.B., Tang, J. & Luo, Y.K. (2016) Evaluation of Liver Ischemia-Reperfusion Injury in Rabbits Using a Nanoscale Ultrasound Contrast Agent Targeting ICAM-1 PLoS One. 11

9.) Shi, J. & Wei, P.K. (2016) Interleukin-8: A potent promoter of angiogenesis in gastric cancer Oncol Lett. 11

10.) Pan, W., Yu, H., Huang, S. & Zhu, P. (2016) Resveratrol Protects against TNF-Alpha-Induced Injury in Human Umbilical Endothelial Cells through Promoting Sirtuin-1-Induced Repression of NF-KB and p38 MAPK PLoS One. 11

11.) Maimaiti, A., Maimaiti, A., Yang, Y. & Ma, Y. (2016) MiR-106b exhibits an anti-angiogenic function by inhibiting STAT3 expression in endothelial cells Lipids Health Dis. 15

12.) Lv, P., Tong, X., Peng, Q., Liu, Y., Jin, H., Liu, R., Sun, W., Pan, B., Zheng, L. & Huang, Y. (2016) Treatment with the herbal medicine, naoxintong improves the protective effect of high-density lipoproteins on endothelial function in patients with type 2 diabetes Mol Med Rep. 13

13.) Liu, X.L., Hu, X., Cai, W.X., Lu, W.W. & Zheng, L.W. (2016) Effect of Granulocyte-Colony Stimulating Factor on Endothelial Cells and Osteoblasts Biomed Res Int. 2016

14.) Liu, K., Wang, X.J., Li, Y.N., Li, B., Qi, J.S., Zhang, J. & Wang, Y. (2016) Tongxinluo Reverses the Hypoxia-suppressed Claudin-9 in Cardiac Microvascular Endothelial Cells Chinese Medical Journal. 129

15.) Hong, H., Jiang, L., Lin, Y., He, C., Zhu, G., Du, Q., Wang, X., She, F. & Chen, Y. (2016) TNF-alpha promotes lymphangiogenesis and lymphatic metastasis of gallbladder cancer through the ERK1/2/AP-1/VEGF-D pathway BMC Cancer. 16

16.) Hong, H., He, C., Zhu, S., Zhang, Y., Wang, X., She, F. & Chen, Y. (2016) CCR7 mediates the TNF-à-induced lymphatic metastasis of gallbladder cancer through the ?ERK1/2 - AP-1? and ?JNK - AP-1? pathways J Exp Clin Cancer Res. 35

17.) He, Y., Luan, Z., Fu, X. & Xu, X. (2016) Overexpression of uncoupling protein 2 inhibits the high glucose-induced apoptosis of human umbilical vein endothelial cells Int J Mol Med. 37

18.) Fan, Y., Qiao, Y., Huang, J. & Tang, M. (2016) Protective Effects of Panax notoginseng Saponins against High Glucose-Induced Oxidative Injury in Rat Retinal Capillary Endothelial Cells Evid Based Complement Alternat Med. 2016

19.) Drolez, A., Vandenhaute, E., Julien, S., Gosselet, F., Burchell, J., Cecchelli, R., Delannoy, P., Dehouck, M.P. & Mysiorek, C. (2016) Selection of a Relevant In Vitro Blood-Brain Barrier Model to Investigate Pro-Metastatic Features of Human Breast Cancer Cell Lines PLoS One. 11

20.) Chen, H., Tang, N., Chen, M. & Chen, D. (2016) Endothelialization of TiO2 Nanorods Coated with Ultrathin Amorphous Carbon Films Nanoscale Research Letters. 11

ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.

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