GeneQuery™ HLA-A*02 Screening Kit by qPCR

The human leukocyte antigen (HLA) complex is a gene complex located on the short arm of human chromosome 6. The human version of the major histocompatibility complex (MHC) helps the immune system distinguish the host cells from invaders. It is the most polymorphic locus in the human genome, and has been implicated in many pathologies including autoimmunity disorders and cancer.

GeneQuery™ HLA-A*02 Screening Kit by qPCR (GSHA02) utilizes SYBR®Green-based qPCR with melt curve analysis to reveal the presence or absence of HLA-A*02 allele in a human cell sample. GSHA02 can utilize either cell lysate or purified genomic DNA as the qPCR template. The cell lysis buffer and enhancer (Cat #GQ400a and GQ400b) are included in the kit to lyse pelleted cell samples. Each GSHA02 kit contains a HLA-A*02 (A02) primer set and a genomic DNA Control (GDC) primer set. The A02 primer set amplifies only the HLA-A*02 allele, but not other HLA-A alleles. The GDC primer set targets a region on human chromosome 21 and is included to examine the quality of the test sample, qPCR components, and qPCR conditions. Each primer set included in GTM-HA26 has been validated by qPCR with melt curve analysis and gel electrophoresis for amplification specificity. The 2X GoldNStart TaqGreen qPCR Master Mix (Cat #MB6018a-1) is a SYBR®Green dye-based qPCR master mix with a “hot-start” property. It contains SYBR®Green, dNTPs, Taq DNA polymerase, and an inert gold-color loading indicator in a single tube. The “hot-start” property achieved through ScienCell’s unique chemically modified Taq DNA polymerase provides maximal inhibition of primer dimer formation. The advanced buffer formulation provides superior specificity and efficiency with a wide linear dynamic range. The inert gold-color loading indicator allows for better visualization and tracking of sample loading in qPCR plates or tubes. The HLA-A*02 positive genomic DNA control (Cat #GK830b) is the gDNA extracted from HLA-A*02 positive cells. The kit provides enough reagents for running 10 qPCR reactions each with the A02 and the GDC primer sets.

Additional Materials Required (Materials Not Included in Kit)