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GeneQuery™ HLA-B PCR+Sanger SBT Typing Kit

Catalog #GK892

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GeneQuery™ HLA-B PCR+Sanger SBT Typing Kit (SBT-B) utilizes the Sequence-Based Typing (SBT) method, which is the gold standard for HLA typing. This method involves PCR amplification of HLA genes, followed by Sanger sequencing of the amplicons. The acquired sequences are aligned to an HLA allele database using the SOAPTyping software (BGI Genomics) to obtain the 4-digit genotypes of the samples. The two haplotypes from both parents can be typed simultaneously using this kit and the SOAPTyping software.

Kit Components:

Cat #   Component Quantity Storage
GK892a HLA-B amplification primer set (B_ampF and B_ampR) 400 µL -20°C
GK892b B_2F sequencing primer mix, 10 µM 200 μL -20°C
GK892c B_2R sequencing primer mix, 10 µM 200 μL -20°C
GK892d B_3F sequencing primer mix, 10 µM 200 μL -20°C
GK892e B_3R sequencing primer mix, 10 µM 200 μL -20°C
GK892f B_4F sequencing primer mix, 10 µM 200 µL -20°C
GK892g B_4R sequencing primer mix, 10 µM 200 μL -20°C
GK892h Nuclease-free H2O 4 mL 4°C
GK892r Human genomic DNA reference (GDR), ~50 ng/µL
(HLA-B*08:01 and HLA-B*18:01)
10 μL -20°C
MB6038-1 2X EmeraldNStart HiFi Marathon PCR Master Mix, 1 mL 2 vials -20°C

Availability: In stock

$595.00
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