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GeneQuery™ HLA-C PCR+Sanger SBT Typing Kit

Catalog #GK893

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GeneQuery™ HLA-C PCR+Sanger SBT Typing Kit (SBT-C) utilizes the Sequence-based Typing (SBT) method, which is the gold standard for HLA typing. This method involves PCR amplification of HLA genes, followed by Sanger sequencing of the amplicons. The acquired sequences are aligned to an HLA allele database using the SOAPTyping software (BGI Genomics) to obtain the 4-digit genotypes of the samples. The two haplotypes from both parents can be typed simultaneously using this kit and the SOAPTyping software.

Kit Components:

Cat #   Component Quantity Storage
GK893a HLA-C amplification primer set (C_ampF and C_ampR) 400 µL -20°C
GK893b C_2F sequencing primer mix, 10 µM 200 μL -20°C
GK893c C_2R sequencing primer mix, 10 µM 200 μL -20°C
GK893d C_3F sequencing primer mix, 10 µM 200 μL -20°C
GK893e C_3R sequencing primer mix, 10 µM 200 μL -20°C
GK893f C_4F sequencing primer mix, 10 µM 200 µL -20°C
GK893g C_4R sequencing primer mix, 10 µM 200 μL -20°C
GK893h Nuclease-free H2O 4 mL 4°C
GK893r Human genomic DNA reference (GDR), ~50 ng/µL
(HLA-C*07:01 and HLA-C*12:03)
10 μL -20°C
MB6038-1 2X EmeraldNStart HiFi Marathon PCR Master Mix, 1 mL 2 vials -20°C

Availability: In stock

$595.00
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