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GeneQuery™ HLA-DPB1 PCR+Sanger SBT Typing Kit

Catalog #GK894

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GeneQuery™ HLA-DPB1 PCR+Sanger SBT Typing Kit (SBT-DPB1) utilizes the Sequence-based Typing (SBT) method, which is the gold standard for HLA typing. This method involves PCR amplification of HLA genes, followed by Sanger sequencing of the amplicons. The acquired sequences are aligned to an HLA allele database using the SOAPTyping software (BGI Genomics) to obtain the 4-digit genotypes of the samples. The two haplotypes from both parents can be typed simultaneously using this kit and the SOAPTyping software.

Kit Components:

Cat #   Component Quantity Storage
GK894a HLA-DPB1 amplification primer set (P_ampF and P_ampR) 400 µL -20°C
GK894b P_2F sequencing primer mix, 10 µM 200 μL -20°C
GK894c P_2R sequencing primer mix, 10 µM 200 μL -20°C
GK894d P_3F sequencing primer mix, 10 µM 200 μL -20°C
GK894e P_3R sequencing primer mix, 10 µM 200 μL -20°C
GK894f P_4F sequencing primer mix, 10 µM 200 µL -20°C
GK894g P_4R sequencing primer mix, 10 µM 200 μL -20°C
GK894h Nuclease-free H2O 4 mL 4°C
GK894r Human genomic DNA reference (GDR), ~50 ng/µL
(HLA-DPB1*01:01 and HLA-DPB1*02:01)
10 μL -20°C
MB6038-1 2X EmeraldNStart HiFi Marathon PCR Master Mix, 1 mL 2 vials -20°C

Availability: In stock

$595.00
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