See How ScienCell Research Laboratories is Proactively Contributing to the Fight Against SARS-CoV-2

GeneQuery™ HLA-DQB1 PCR+Sanger SBT Typing Kit

Catalog #GK895

Be the first to review this product

GeneQuery™ HLA-DQB1 PCR+Sanger SBT Typing Kit (SBT-DQB1) utilizes the Sequence-based Typing (SBT) method, which is the gold standard for HLA typing. This method involves PCR amplification of HLA genes, followed by Sanger sequencing of the amplicons. The acquired sequences are aligned to an HLA allele database using the SOAPTyping software (BGI Genomics) to obtain the 4-digit genotypes of the samples. The two haplotypes from both parents can be typed simultaneously using this kit and the SOAPTyping software.

Kit Components:

Cat #   Component Quantity Storage
GK895a HLA-DQB1 amplification primer set (Q_ampF and Q_ampR) 400 µL -20°C
GK895b Q_2F sequencing primer mix, 10 µM 200 μL -20°C
GK895c Q_2R sequencing primer mix, 10 µM 200 μL -20°C
GK895d Q_3F sequencing primer mix, 10 µM 200 μL -20°C
GK895e Q_3R sequencing primer mix, 10 µM 200 μL -20°C
GK895f Q_4F sequencing primer mix, 10 µM 200 µL -20°C
GK895g Q_4R sequencing primer mix, 10 µM 200 μL -20°C
GK895h Nuclease-free H2O 4 mL 4°C
GK895r Human genomic DNA reference (GDR), ~50 ng/µL
(HLA-DQB1*02:01 and HLA-DQB1*06:02)
10 μL -20°C
MB6038-1 2X EmeraldNStart HiFi Marathon PCR Master Mix, 1 mL 2 vials -20°C

Availability: In stock

$595.00
Product Description Specifications Technical Resources Product Reviews Publications

Back to top