ScienCell's GeneQuery™ Human Alzheimer’s Disease qPCR Array Kit (GQH-ALZ) facilitates gene expression profiling of key genes involved in Alzheimer’s disease onset and progression.  Alzheimer’s disease is a neurodegenerative disorder that results in progressive memory loss, disorientation, impaired thinking, and change in personality.  The disease exists in both early-onset and late-onset forms with progression characterized by amyloid plaque formation.  This kit focuses on late-onset Alzheimer’s although some early-onset susceptibility genes are included.  Brief examples of how included genes may be characterized are shown below:

• Late-onset risk factors: APOE, CLU, CR1, PAXIP1, PICALM
• Early-onset risk factors: PSEN1, PSEN2, APP, SORL1
• Amyloid plaque formation: APP, APBB1, APBB2, RELN, BLMH
• Disease progression/biomarkers: NOS3, GSK3B, HFE, PLAU, ACE

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg²⁺and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.