GeneQuery™ Human Autism Spectrum Disorder qPCR Array Kit

ScienCell’s GeneQuery™ Human Autism Spectrum Disorder qPCR Array Kit (GQH-ASD) profiles 88 key genes associated with Autism Spectrum Disorder. Autism Spectrum Disorder is a developmental disability that can result in social, communication, behavioral, and learning challenges. The disease is early-appearing, with symptoms often presenting early in childhood, and evidence suggests there is a strong hereditary component. Brief examples of how included genes may be grouped according to their functions are shown below:

• Neurodevelopment: ADNP, ARID1B, CHD2, DISC1, DSCAM, FOXP1, KMT2C, RIMS, TCF4, ZBTB20, ANKRD11, ASH1L, ASXL3, AUTS2, BCL11A, CACNA1H, GRIN2B, KATNAL2, KDM6B, MET, NCKAP1, NRXN3, PHF2, POGZ, RBFOX1, RELN, SEMA5A, SHANK2
• Impaired communication/behavioral ability: CNTNAP2, FOXP2, GRIK2, KMT2A, MACROD2, MED13L, PAX5, PTEN, SHANK3, SPAST, KMT5B
• Social deficits/behavioral abnormalities: AVPR1A, BCKDK, CNTNAP4, DIP2A, GRIP1, HMGN1
• Calcium signaling/calcium channels: ATP2B2, CACNB2, CDC42BPB, PRKCB
• Altered expression linked to ASD: FOXP1, GABRB3, TRIO, ZMYND11, CHRNA7, KDM5B, SETBP1, SYNGAP1, TBR1, TRIP12, WAC, WDFY3, ASTN2, CACNA2D3, CHD8, CTCF, CTTNBP2, GPHN, GRIP1, MBD5, MYT1L, NLGN3, NR3C2, NRXN1, PTCHD1, SETD5
• Novel variants found in ASD: DEAF1, MAGEL2, TNRC6B, TRPC6
• Other risk factors for Autism: CACNA1D, CHD8, CUL3, MACROD2, SCN2A, OXTR, SCN2A, SLC9A9, CTNND2, MECP2
• Linked to non-syndromic autism: ANK2, KMT2E, NLGN4X, TRPC6, SHANK3

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.