GeneQuery™ Human B Cell Receptor Signaling Pathway qPCR Array Kit

ScienCell's GeneQuery™ Human B Cell Receptor Signaling Pathway qPCR Array Kit (GQH-BRS) is designed to facilitate gene expression profiling of 88 key genes involved in B cell receptor signaling pathway. The B cell receptor signaling pathway is important for antibody production, B cell proliferation and differentiation. Brief examples of how included genes may be grouped according to their functions are shown below:     

• B cell receptor complex components: CD79A, CD79B
• Co-inhibitory receptors: CD22, CD72, FCGR2B
• Co-stimulators: CD81, CR2, IFITM1
• Expression adaptor proteins: BLNK, GAB2, GRB2, CARD11
• Kinases: LYN, SYK, PI3Ks
• Phosphatases: INPP5D, PTPN6
• MAPK pathway: KRAS, HRAS, NRAS, RAF1, MAP2K1, MAPK1, MAPK3
• PI3K/AKT pathway: PI3Ks, AKT1, AKT2
• IKK/NFKB pathway: NFKBs, PRKCB, PLCG2, DAG1, BCL10, CARD11, MAP3K7, MALT1, CHUK, IKBKB, IKBKG

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg²⁺, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.