GeneQuery™ Human Breast Cancer qPCR Array Kit

ScienCell’s GeneQuery™ Human Breast Cancer qPCR Array Kit (GQH-BRC) profiles 88 key genes associated with the development and progression of breast cancer. Breast cancer is a disease that occurs when cells in the breast begin to proliferate at a rapid and uncontrolled growth rate. This type of cancer is highly prevalent, serving as the most common cancer in women worldwide and affecting approximately 1 in 8 women. Breast cancer has the second highest cancer mortality rate among women, though this rate is slowly declining as a result of improved treatment and early detection. While there are many factors that contribute to the development of this disease, studies have found an increasing number of genetic factors can influence an individual’s overall susceptibility, metastatic potential, prognostic outlook, and treatment options. Brief examples of how included genes may be grouped according to their functions are shown below:

• Susceptibility/Risk Indicator: ATM, BARD1, BRCA1, BRCA2, CCND1, CHEK2, ESR1, FANCM, MLH1, MSH2, MSH6, MUTYH, PALB2, PIK3CA, PTEN, RAD51B, RAD51C, RAD51D, STK11, TOX3, TP53, XRCC1
• Prognostic Indicator: ALDH1A1, CD24, CD44, CDC73, E2F1, HMGB2, HOXA5, HSD17B4, IL17RB, MAP3K1, MYC, NOTCH1, SIRT7, SNAI2
• Overexpressed in Breast Cancer: ACKR3, CDC25A, ERBB2, HOXB7, KIF11, MDM2, MDM4, PML, STAT3, TWIST1
• Therapeutic Target: BRD4, CD47, CIB1, EN1, FOXA1, RB1, SLC2A1, SLFN11, USP17L2, WT1
• Associated with Metastasis: ALDH1A3, CARM1, CDH1, CXCR4, GATA3, HMGB1, NOTCH3, PDGFRA, PPFIA1, RBMS3, SNAI1, SQSTM1, UBR5
• Associated with Treatment Resistance: APC, CXCL12, EGFR, MET, SOX2, SOX9, ZEB1
• Associated with Aggressive Phenotypes: ANP32E, CD274, JPT2, MKI67, MUC1, NFE2L2, TERT
• Tumor Suppressor Gene: ADAM23, CDKN1B, MEN1
• Breast Cancer Oncogene: KDM2B, RUNX1

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.