GeneQuery™ Human Cyclins and Cyclin-dependent Kinases qPCR Array Kit

ScienCell's GeneQuery™ Human Cyclins and Cyclin-dependent Kinases qPCR Array Kit (GQH-CCN) is designed to facilitate gene expression profiling of 40 selected cyclins and cyclin-dependent kinases (CDKs). CDKs are a family of small enzymes required for cell cycle progression and cell division, by means of phosphorylation of their substrates. CDKs must bind to cyclins to become fully activated. Cyclin/CDK complexes regulate cell cycle checkpoints and are the driving force of cell cycle progression. Brief examples of how included genes may be grouped are shown below:

• Cyclins: CCNA, CCNB, CCNC, CCND, CCNE, CCNF, CCNG, CCNH, CCNI, CCNK, CCNL, CCNO, CCNT, CCNY
• CDKs: CDK1, CDK2, CDK3, CDK4, CDK5, CDK6, CDK7, CDK8, CDK10, CDK20

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg²⁺, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.