GeneQuery™ Human Diabetes, Type II (Late-onset, Insulin Resistance) qPCR Array Kit

ScienCell's GeneQuery™ Human Diabetes, Type II (Late-onset, Insulin Resistance) qPCR Array Kit (GQH-DB2) is designed to facilitate gene expression profiling of 88 key genes involved in the development and progression of late-onset diabetes mellitus. Type 2 diabetes mellitus is characterized by abnormally high blood sugar levels resulting from a failure to utilize insulin properly. Brief examples of how included genes may be grouped according to function are shown below:

• Blood glucose regulation: INS, INSR, SLC2A2, SLC30A8, ABCC8
• Fat regulation: ADIPOQ, LPL, PON1, LEP, RETN
• Cholesterol regulation: APOA1, APOB, LPA, CETP
• Metabolism: UCP2, GCG, PCK2, GYS1

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg²⁺ and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.