GeneQuery™ Human Endothelial Cell Heterogeneity qPCR Array Kit

ScienCell's GeneQuery™ Human Endothelial Cell Heterogeneity qPCR Array Kit (GQH-ECH) is designed to facilitate gene expression profiling of 88 key genes that are differentially expressed in vascular endothelial cells, and in endothelial cells from various tissues. Brief examples of how included genes may be grouped according to vascular types and tissue origins are shown below:     

• Endothelial cells vs. non-endothelial cells: PECAM1, CDH5, MMRN1, EFEMP1, VWF
• Artery cells vs. vein cells: ALDH1A1, MPZL2, LIPG, ITM2A, CD44, MYO1B, GDF1, LEFTY1, SMO, EPHB4
• Large vessel cells vs. microvessel cells: SPARC, JAM3, VEGFC, COL5A1, ROBO1, MYLK, COL4A3BP, EDNRB, VAV3, PDGFD
• Tissue origin:
• skin: FGF2, SCD, HMGCS1
• lung: SFRP1, OGN
• uterus: GAL
• intestine: BTD

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg²⁺, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.