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- GeneQuery™ Human Hepatic Stellate Cell Biology qPCR Array Kit
ScienCell's GeneQuery™ Human Hepatic Stellate Cell Biology qPCR Array Kit (GQH-HSB) is designed to facilitate gene expression profiling of 88 key genes involved in (i) human hepatic stellate cell activation and proliferation; (ii) retinol metabolism; (iii) extracellular matrix (ECM) synthesis and remodeling; and (iv) hepatic stellate cell related disorders. Brief examples of how included genes may be grouped according to their functions are shown below:
- Hepatic stellate cell markers: ACTA2, GFAP, DES, NGFR, COL2A1, VIM, LAMA1, NES
- Hepatic stellate cell activation and proliferation: ICAM1, MMP13, RELN, TIMP1, SYP, CDH1, RARA, PDGFs, TGFB1, MMP2
- Liver development and regeneration: STX2, PTN, SYNM, FOXF1, ICAM1, VCAM1, ITGB3, HGF, CXCL12, DES
- Retinol metabolism: RBP1, RBP2, RBP4, RBP5, CRABP1, CRABP2, LRAT, CYP26
- ECM synthesis and remodeling: TGFB1, COL4A2, Laminin subunits, SPARC, VCAN, CTGF
- Cytokine production: EGF, IGFs, PDGFs, FGFs, CSF1, CCL2, TNF, CXCL2, CXCL3, CXCL8
- Hepatic fibrosis and cancer development: PKHD1, TMEM67, RPGRIP1L, SLC17A5, PKD1, CC2D2A, B9D1, B9D2
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
Catalog No. | GK091 |
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Country of Manufacture | United States |
Product Code | GQH-HSB |
Size/Quantity | One 96-well plate, one 20ul reaction per well |
Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
Storage | Store at 4°C upon receipt. For long-term storage (>1 year), store the plate at -20°C in a manual defrost freezer. |
Shipping Info | Ambient temperature. |
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