GeneQuery™ Human Non-Small Cell Lung Cancer qPCR Array Kit

ScienCell's GeneQuery™ Human Non-Small Cell Lung Cancer qPCR Array Kit (GQH-NLC) is designed to facilitate gene expression profiling of 88 key genes involved in non-small cell lung cancer (NSCLC) carcinogenesis. NSCLC accounts for more than 80% of all lung cancers. There are three main types of NSCLC: adenocarcinoma, lung squamous cell carcinoma and lung large cell carcinoma. Brief examples of how included genes may be grouped according to their functions are shown below:     

• Oncogenes and tumor suppressor genes: KRAS, HRAS, EML4, ALK, RARB, FHIT, RASSF1, TP53, CDKN2A
• Cell cycle regulation and apoptosis: CDK4, CDK6, CCND1, CCNE2, CASP3, CASP9, BAD, STK4, FOXO3
• MAPK pathway: KRAS, HRAS, NRAS, RAF1, MAP2K1, MAPK1, MAPK3
• PI3K/AKT pathway: PI3Ks, AKT1, AKT2
• Genes related to specific lung cancer types
• Adenocarcinoma: ACTR3B, CADM1, ERBB2, CALB2, CDH1, EGF, FIGF, KRT20, NKX2-1, OGG1
• Lung squamous cell carcinoma: CDH10, CYP1A1, FAM83B, FGFR1, GRAMD4, KRT19, PEBP1, PRKCI, S100A2, SOX2
• Lung large cell carcinoma: CASK, CDC42BPB, DDR2, EPHA3, MYO18B, NME1, ROS1, TTN, WNK3

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg²⁺, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.