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- GeneQuery™ Human Smooth Muscle Contraction and Diseases qPCR Array Kit
ScienCell's GeneQuery™ GQH-SMD qPCR Array is designed to profile the gene expression of 88 key genes involved in smooth muscle contraction and diseases that affect smooth muscle function. Vertebrates have three main types of muscle tissue: cardiac, skeletal, and smooth or non-striated. Smooth muscle fundamentally differs from the other two types in both regulation and function. Smooth muscle contraction is involuntary and it is found lining blood vessels, lymphatic vessels, the bladder, and the reproductive, GI, and respiratory tracts. Smooth muscle malfunctions can lead to a host of disorders such as achalasia (when smooth muscles fail to relax) and Hirschsprung’s (muscle failure in the GI tract due to lack of nerves). Brief examples of how included genes may be grouped according to relevance are shown below:
- Smooth muscle contraction: SORBS3, TPM1, MYL9, ITGA1, CALM1, ACTA2
- Smooth muscle differentiation: CAMK2D, KCNH8, KLF4, MEF2A, SRF
- Achalasia: RET, NOS1, KIT, FLVCR1, MC2R
- Hirschsprung’s disease: ECE1, EDN3, EDNRB, GDNF, SOX10
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
Catalog No. | GK067 |
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Country of Manufacture | United States |
Product Code | GQH-SMD |
Size/Quantity | One 96-well plate, one 20ul reaction per well |
Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
Storage | Store at 4°C upon receipt. For long-term storage (>1 year), store the plate at -20°C in a manual defrost freezer. |
Shipping Info | Ambient temperature. |
References | [1] Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F. (2002) "Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes." Genome Biol. 3(7): 1-12. |
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1100 | HBVSMC | Human Brain Vascular Smooth Muscle Cells |
$680.00
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2710 | HEsSMC | Human Esophageal Smooth Muscle Cells |
$726.00
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2810 | HGSMC | Human Gastric Smooth Muscle Cells |
$726.00
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2940 | HCoSMC | Human Colonic Smooth Muscle Cells |
$726.00
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3110 | HPASMC | Human Pulmonary Artery Smooth Muscle Cells |
$680.00
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3400 | HBSMC | Human Bronchial Smooth Muscle Cells |
$726.00
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$693.00
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6114 | HASMC cDNA | Human Aortic Smooth Muscle Cell cDNA |
$411.00
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2814 | HGSMC cDNA | Human Gastric Smooth Muscle Cell cDNA |
$411.00
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2944 | HCoSMC cDNA | Human Colonic Smooth Muscle Cell cDNA |
$411.00
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3114 | HPASMC cDNA | Human Pulmonary Artery Smooth Muscle Cell cDNA |
$411.00
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||
3404 | HBSMC cDNA | Human Bronchial Smooth Muscle Cell cDNA |
$411.00
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MEDIA | |||||
1101 | SMCM | Smooth Muscle Cell Medium |
$114.00
As low as:
$78.00
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MOLECULAR BIOLOGY | |||||
1104 | HBVSMC cDNA | Human Brain Vascular Smooth Muscle Cell cDNA |
$411.00
|