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ScienCell recommends using at least 4 controls: 1 internal control to normalize your data (e.g. β-actin, GAPDH, LDHA, NONO, and PPIH) 1 DNA quality control to detect genomic DNA contamination in your cDNA samples (e.g. GDC, a primer set that targets a non-transcribed region of the genome 1 positive control (ScienCell offers PPC, which is pre-dispensed synthetic DNA sequence not present in the human genome with a primer pair that can amplify it) 1 negative control (a well with qPCR primers in which you add all reagents except the template DNA

Controls
select individual genes or

   Beta-actin    B2M    GAPDH    GUSB    HPRT1    HSP90AB1
   LDHA    NONO    PGK1    PPIH    RPLP0    TFRC
   GDC    PPC


Please note that these filters reflect the gene primers we currently have in stock only. If you notice that your favorite gene is missing, just request it in the Additional Comments or Requests section before requesting your primers.


 

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