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How can we help you today?
Need help with product selection and ordering?
Email us at sales@sciencellonline.com
Need help with scientific inquiries, technical assistance, product applications, and use?
Email us at techsupport@sciencellonline.com
Need help with issues concerning this website?
Email us at webmaster@sciencellonline.com
Have any marketing related inquiries?
Email us at marketing@sciencellonline.com
Need help ordering from abroad?
As the largest cell provider, we are available worldwide.
Please visit our Distributors page to select the company nearest to your location.
We are located at 1610 Faraday Ave, Carlsbad, CA 92008
Call us at (877) 602-8549 or (760) 602-8549
Fax us at (760) 602-8575
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The adrenal gland plays an essential role in regulating homeostasis in the body through the secretion of corticosteroid and androgen hormones. Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies, as they are one of the easiest types of cells to grow in culture. Their durability makes them amenable to a variety of manipulations ranging from gene transfection to microinjection. Fibroblasts secrete a non-rigid extracellular matrix that is rich in type I and/or type III collagen. They are responsible for much of the synthesis of extracellular matrix in connective tissues and play a central role in wound healing. Many diseases are associated with fibroblasts, either because fibroblasts are implicated in their etiology or because of the fibrosis that accompanies damage to other cell types in tissues. For example, the development of bowel stenosis in Crohn’s disease patients is caused by extreme fibroblast proliferation and extracellular matrix expansion.
HAdF from ScienCell Research Laboratories are isolated from human adrenal tissue. HAdF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HAdF are characterized by their spindle morphology and immunofluorescence with antibody specific to fibronectin. HAdF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HAdF are guaranteed to further expand for 15 population doublings under the
conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium (FM, Cat. No. 2301) for the culturing of HAdF in vitro
Catalog No. | 3630 |
---|---|
Country of Manufacture | United States |
Product Code | HAdF |
Size/Quantity | 5 x 10^5 cells/vial |
Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. |
Shipping Info | Dry ice. |
References | [1] Conrad GW, Hart GW, Chen Y. (1977) "Differences in vitro between fibroblast-like cells from cornea, heart,
and skin of embryonic chicks." J. Cell Sci. 26: 119-37. [2] Gabbiani G, Rungger-Brandle E. (1981) "The fibroblast." In Glynn LE, Handbook of Inflammation, Vol. 3: In Tissue Repair and Regeneration (pp. 1-50) Amsterdam: Elsevier. [3] Luna J, Masamunt, MC, Rickmann M, Mora R, Espana C, Delgado S, Llach, J, Vaquero E, Sans M. (2011) "Rocotrienols have potent antifibrogenic effects in human intestinal fibroblasts." Inflamm Bowel Dis. 17: 732-41. |
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We are an expanding biotechnology company whose mission is the research and development of cell and cell-related products for experimental use. About Sciencell