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- Human Aortic Adventitial Fibroblasts
In vascular adventitia, the outermost connective tissue covering the vessel, adventitial fibroblasts (AF) produce collagen to provide structural support by anchoring the blood vessel to nearby tissues. AF are the first cells of the vascular wall to respond to hypertension and vascular injury through activation and proliferation. During pathological conditions, AF produce cytokines and chemokines to induce mass infiltration of immune cells into the adventitial layer of the vessel wall. Immune cell infiltration into the adventitia results in adventitial inflammation and can lead to cardiovascular disease. The important properties of AF make AF cultures an ideal tool for studying the pathogenesis of cardiovascular disease and for the development of novel disease treatments.
HAAF from ScienCell Research Laboratories are isolated from human aortic artery. HAAF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HAAF are characterized by spindle morphology and by immunofluorescence with antibody specific to fibronectin. HAAF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HAAF are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium-2 (FM-2, Cat. #2331) for culturing HAAF in vitro.
| Catalog No. | 6120 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HAAF |
| Size/Quantity | 5 x 10^5 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Conrad, G. W., Hart, G. W., Chen, Y. (1977) Differences in vitro between fibroblast-like cells from cornea, heart, and skin of embryonic chicks. J. Cell Sci. 26:119-137. [2] Pendurthi, U. R., Rao, L. V. M., Williams, J. T. and Idell, S. (1999) Regulation of tissue factor pathway inhibitor expression in smooth muscle cells. Blood 94:579-586. [3] Das M, Dempsey EC, Reeves JT, Stenmark KR. (2002) Selective expansion of fibroblast subpopulations from pulmonary artery adventitia in response to hypoxia. Am J Physiol Lung Cell Mol Physiol 282(5):L976-86. |
61201.) Guo T, Fang M, Zhang D, Li X. (2013) "Combination treatment with asiaticoside and rapamycin: A new hope for in?stent restenosis." ExpTher Med.6: 557
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| 6124 | HAF cDNA | Human Aortic Fibroblast cDNA |
$453.00
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| 6125 | HAF tRNA | Human Aortic Adventitial Fibroblast Total RNA |
$401.00
|
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| 6126 | HAF Lysate | Human Aortic Adventitial Fibroblast Lysate |
$438.00
|
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| 6127 | HAF miRNA | Human Aortic Fibroblast MicroRNA |
$374.00
|
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| 6129 | HAF gDNA | Human Aortic Adventitial Fibroblast Genomic DNA |
$438.00
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