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- Human Aortic Smooth Muscle Cells
Smooth muscle cells (SMC) are primary contributors to the development of arterial disease. The ability of vascular SMC to switch to a proliferative phenotype is one of the main factors in the development and progression of vascular disease. Recent studies have demonstrated that SMC express calcium channels, ICAM-1, and VCAM-1. The expression of ICAM-1 and VCAM-1 on SMC may contribute to the inflammatory reaction in the vascular wall and may actively be involved in the progression of vascular disease. Vascular SMC in culture play an important role in vascular disease research and can be used to identify new therapeutic targets to treat arterial disease.
HASMC from ScienCell Research Laboratories are isolated from human aorta. HASMC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HASMC are characterized by immunofluorescence with antibodies to specific to α-smooth muscle actin. HASMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HASMC are guaranteed to further expand for 15 population doublings under the conditions specified by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Smooth Muscle Cell Medium (SMCM, Cat. #1101) for culturing HASMC in vitro.
Catalog No. | 6110 |
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Country of Manufacture | United States |
Product Code | HASMC |
Size/Quantity | 5 x 10⁵ cells/vial. |
Product Use | HASMC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures. |
Storage | Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments. |
Shipping Info | Dry ice. |
References | [1] Schwartz, S. M., Campbell, G. R., Campbell, J. H. (1986) Replication of smooth muscle cells in vascular disease. Circ. Res. 58:427-444. [2] Fan, Q. I., Vanderpool, K., Marsh, J. D. (2002) A 27 bp cis-acting sequence is essential for L-type calcium channel alpha(1C) subunit expression in vascular smooth muscle cells. Biochim Biophys Acta. 1577:401-11. [3] Braun, M., Pietsch, P., Schror, K., Baumann, G., Felix, S. B. (1999) Cellular adhesion molecules on vascular smooth muscle cells. Cardiovasc. Res. 41:395-401. |
1.) Pan, Y., Zhao, G., Cai, Z., Chen, F., Xu, D., Huang, S., Lan, H. & Tong, Y.(2016) 'Synergistic Effect of Ferulic Acid and ZLigustilide, Major Components of A. sinensis, on Regulating ColdSensing Protein TRPM8 and TPRA1 In Vitro' Evid Based Complement Alternat Med. VOL 2016
2.) Kim, Y.A., Lee, D.G., Yi, K.Y., Lee, B.H. & Jung, Y.S.(2016) 'Blockade of Urotensin II Receptor Prevents Vascular Dysfunction' Biomolecules & Therapeutics. VOL 24
3.) Zheng, C.Y., Song, L.L., Wen, J.K., Li, L.M., Guo, Z.W., Zhou, P.P., Wang, C., Li, Y.H., Ma, D. & Zheng, B. (2015) Tongxinluo (TXL), a Traditional Chinese Medicinal Compound, Improves Endothelial Function After Chronic Hypoxia Both In Vivo and In Vitro J Cardiovasc Pharmacol. 65
4.) Park, S.H., Sung, Y.Y., Nho, K.J. & Kim, H.K. (2015) Protective activity ethanol extract of the fruits of Illicium verum against atherogenesis in apolipoprotein E knockout mice BMC Complement Altern Med. 15
5.) Pahl, M.C., Erdman, R., Kuivaniemi, H., Lillvis, J.H., Elmore, J.R. & Tromp, G. (2015) Transcriptional (ChIP-Chip) Analysis of ELF1, ETS2, RUNX1 and STAT5 in Human Abdominal Aortic Aneurysm Int J Mol Sci. 16
6.) Li, J., Zhou, J., Zhang, D., Song, Y., She, J. & Bai, C. (2015) Bone marrow-derived mesenchymal stem cells enhance autophagy via PI3K/AKT signalling to reduce the severity of ischaemia/reperfusion-induced lung injury J Cell Mol Med. 19
7.) Koo, H.J., Sohn, E.H., Pyo, S., Woo, H.G., Park, D.W., Ham, Y.M., Jang, S.A., Park, S.Y. & Kang, S.C. (2015) An ethanol root extract of Cynanchum wilfordii containing acetophenones suppresses the expression of VCAM-1 and ICAM-1 in TNF-à-stimulated human aortic smooth muscle cells through the NF-?B pathway Int J Mol Med. 35
8.) Guo T, Fang M, Zhang D, Li X. (2013) "Combination treatment with asiaticoside and rapamycin: A new hope for in?stent restenosis." ExpTher Med.6: 557
9.) Liu F, Zhong H, Liang JY, Fu P, Luo ZJ, Zhou L, Gou R, Huang J. (2010) "Effect of high glucose levels on the calcification of vascular smooth muscle cells by inducing osteoblastic differentiation and intracellular calcium deposition via BMP-2/Cbf?-1 pathway." J Zhejiang Univ Sci B. 11: 905-11.
10.) Xue JH, Yuan Z, Wu Y, Liu Y, Zhao Y, Zhang WP, Tian YL, Liu WM, Liu Y, Kishimoto C. (2010) "High glucose promotes intracellular lipid accumulation in vascular smooth muscle cells by impairing cholesterol influx and efflux balance." Cardiovasc Res. 86: 141-50.
11.) Geng J, Zhao Z, Kang W, Wang W, Liu G, Sun Y, Zhang Y, Ge Z. (2009) "Hypertrophic response to angiotensin II is mediated by protein kinase D-extracellular signal-regulated kinase 5 pathway in human aortic smooth muscle cells." Biochem Biophys Res Commun. 388: 517-22.
12.) Shin IS, Kim JM, Kim KL, Jang SY, Jeon ES, Choi SH, Kim DK, Suh W, Kim YW. (2009) "Early growth response factor-1 is associated with intraluminal thrombus formation in human abdominal aortic aneurysm." J Am Coll Cardiol. 53: 792-9.
13.) Heo SK, Yun HJ, Noh EK, Park WH, Park SD. (2008) "LPS induces inflammatory responses in human aortic vascular smooth muscle cells via Toll-like receptor 4 expression and nitric oxide production." Immunol Lett. 120: 57-64.
14.) Zhang X, Baughman CB, Kaplan DL. (2008) "In vitro evaluation of electrospun silk fibroin scaffolds for vascular cell growth." Biomaterials. 29: 2217-27.
15.) Song Y, Qi H, Wu C. (2007) "Effect of 1,25-(OH)2D3 (a vitamin D analogue) on passively sensitized human airway smooth muscle cells." Respirology. 12: 486-94.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
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CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
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MEDIA | |||||
1101 | SMCM | Smooth Muscle Cell Medium |
$114.00
As low as:
$78.00
|
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REAGENTS | |||||
0103 | T/E | Trypsin/EDTA Solution, 0.25% |
$21.00
|
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0113 | TNS | Trypsin Neutralization Solution, 100 ml |
$21.00
|
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0303 | DPBS | Dulbecco's Phosphate-Buffered Saline, 500 ml |
$16.00
|
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0403 | PLL | Poly-L-Lysine, 1 mg/ml |
$16.00
|
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0953 | SMCF | SMCFectagen |
$253.00
|
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MOLECULAR BIOLOGY | |||||
6114 | HASMC cDNA | Human Aortic Smooth Muscle Cell cDNA |
$411.00
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6115 | HASMC tRNA | Human Aortic Smooth Muscle Cell Total RNA |
$364.00
|
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6116 | HASMC Lysate | Human Aortic Smooth Muscle Cell Lysate |
$398.00
|
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6117 | HASMC miRNA | Human Aortic Smooth Muscle Cell MicroRNA |
$340.00
|
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6119 | HASMC gDNA | Human Aortic Smooth Muscle Cell genomic DNA |
$398.00
|