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Astrocytes are the major cell type in the mammalian brain. They provide a variety of supportive functions to their partner neurons in the central nervous system (CNS), such as neuronal guidance during development, and nutritional and metabolic support throughout life. Astrocytes have also been implicated in various pathological processes. Impairment of normal astrocyte functions during stroke and other insults can critically influence neuron survival. Long-term recovery after brain injury, through neurite outgrowth, synaptic plasticity, or neuron regeneration, is also influenced by astrocyte surface molecule expression and trophic factor release. Numerous studies have demonstrated that astrocytes are among the most functionally diverse group of cells in the CNS. Much of what we have learned about astrocytes is from in vitro studies and astrocyte culture is a useful tool for exploring the diverse properties of this cell type.
HA from ScienCell Research Laboratories are isolated from human brain (cerebral cortex). HA are cryopreserved at passage one and delivered frozen. Each vial contains >1 x 10⁶ cells in 1 ml volume. HA are characterized by immunofluorescence with antibody specific to GFAP. HA are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HA are guaranteed to further expand for 10 population doublings under the conditions provided by ScienCell Research
Laboratories.
Recommended Medium
It is recommended to use Astrocyte Medium (AM, Cat. #1801) for culturing HA in vitro.
| Catalog No. | 1800 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HA |
| Size/Quantity | 1 x 10⁶ cells/vial. |
| Product Use | HA are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures. |
| Storage | Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] G. I. Hatton (2002) Glial-neuronal interactions in the mammalian brain. Adv. in Physiol. Edu. 26:225-237. [2] Van der Laan, L. J. W., De Groot, C. J. A., Elices, M. J. and Dijkstran, C. D. (1997) Extracellular matrix proteins expressed by human adult astrocytes in vivo and in vitro: an astrocyte surface protein containing the CS1 domain contributes to binding of lymphoblasts. J. Neurosci. Res. 50:539-548. [3] Chen Y., and Swanson, R. A. (2003) Astrocytes and brain injury. J. Cereb. Blood Flow Metab. 23:137-149. |
Product Sheets
1800-5 (5 Million Cells per Vial)
1800-10 (10 Million Cells per Vial)
Certificates of Analysis
For a specific lot number, please search below:
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Healthy Robust Astrocytes Review by Carrie
Ease-of-use Overall quality of the product Technical resources These cells are very healthy and GFAP ADH1L1 positive. The protocols are well written and easy to follow. Customer support for the product is also good. (Posted on 7/28/2021)
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Best I've Seen Review by Hamed
Ease-of-use Overall quality of the product Technical resources These astrocytes with the media have been the best I have worked with. Highly recommended, plus the team is super friendly talking to them on the phone. (Posted on 12/21/2019)
1.) Cynthia L. Winham, Timmy Le, Evan R. Jellison, Adam C. Silver, Aime A.Levesque, Andrew O.Koob (2019) y-Synuclein Induces Human Cortical Astrocyte Proliferation and Subsequent BDNF Expression and Release.
2.) Yin JC, Zhang L, Ma NX, Wang Y, Lee G, Hou XY, Lei ZF, Zhang FY, Dong FP, Wu GY, Chen G. (2019). Chemical Conversion of Human Fetal Astrocytes into Neurons through Modulation of Multiple Signaling Pathways. 12(3):488-501.
3.) Morgan SV, Garwood CJ, Jennings L, Simpson JE, Castelli LM, Heath PR, Mihaylov SR, Vaquez-Villaseeor I, Minshull TC, Ince PG, Dickman MJ, Hautbergue GM, Wharton SB. (2018). Proteomic and cellular localisation studies suggest non-tight junction cytoplasmic and nuclear roles for occludin in astrocytes. Eur J Neurosci. 47(12):1444-1456.
4.) Zhou, Y., Zhang, H., Zheng, B., Ye, L., Zhu, S., Johnson, N.R., Wang, Z., Wei, X., Chen, D., Cao, G., Fu, X., Li, X., Xu, H.Z. & Xiao, J. (2016) Retinoic Acid Induced-Autophagic Flux Inhibits ER-Stress Dependent Apoptosis and Prevents Disruption of Blood-Spinal Cord Barrier after Spinal Cord Injury Int J Biol Sci. 12
5.) Shang, C., Hong, Y., Guo, Y. & Xue, Y. (2016) Mir-338-3p Inhibits Malignant Biological Behaviors of Glioma Cells by Targeting MACC1 Gene Med Sci Monit. 22
6.) Olsson, M., Hultman, K., Dunoyer-Geindre, S., Curtis, M.A., Faull, R.L.M., Kruithof, E.K.O. & Jern, C. (2016) Epigenetic Regulation of Tissue-Type Plasminogen Activator in Human Brain Tissue and Brain-Derived Cells Gene Regul Syst Bio. 10
7.) Liang, Y., Xie, X., Chen, L., Yan, S., Ye, X., Anjum, K., Huang, H., Lian, X. & Zhang, Z. (2016) Bioactive Polycyclic Quinones from Marine Streptomyces sp. 182SMLY Mar Drugs. 14
8.) Herland, A., van der Meer, A.D., FitzGerald, E.A., Park, T.E., Sleeboom, J.J.F. & Ingber, D.E. (2016) Distinct Contributions of Astrocytes and Pericytes to Neuroinflammation Identified in a 3D Human Blood-Brain Barrier on a Chip PLoS One. 11
9.) Zhao, H.F., Wang, J., Jiang, H.R., Chen, Z.P. & To, S.S.T.(2016) 'PI3K p110? isoform synergizes with JNK in the regulation of glioblastoma cell proliferation and migration through Akt and FAK inhibition' J Exp Clin Cancer Res. VOL 35
10.) Revathikumar, P., Bergqvist, F., Gopalakrishnan, S., Korotkova, M., Jakobsson, P.J., Lampa, J. & Le Ma?tre, E.(2016) 'Immunomodulatory effects of nicotine on interleukin 1? activated human astrocytes and the role of cyclooxygenase 2 in the underlying mechanism' J Neuroinflammation. VOL 13
11.) O'Sullivan, S.A., Gasparini, F., Mir, A.K. & Dev, K.K.(2016) 'Fractalkine shedding is mediated by p38 and the ADAM10 protease under proinflammatory conditions in human astrocytes' J Neuroinflammation. VOL 13
12.) Kenig, S., Faoro, V., Bourkoula, E., Podergajs, N., Ius, T., Vindigni, M., Skrap, M., Lah, T., Cesselli, D., Storici, P. & Vindigni, A.(2016) 'Topoisomerase II? mediates the resistance of glioblastoma stem cells to replication stressinducing drugs' Cancer Cell Int. VOL 16
13.) Jiang, P., Chen, C., Liu, X.B., Pleasure, D.E., Liu, Y. & Deng, W.(2016) 'Human iPSCderived Immature Astroglia Promote Oligodendrogenesis by increased TIMP1 Secretion' Cell reports. VOL 15
14.) Gu, J.J., Zhang, J.H., Chen, H.J. & Wang, S.S.(2016) 'MicroRNA130b promotes cell proliferation and invasion by inhibiting peroxisome proliferatoractivated receptory in human glioma cells' Int J Mol Med. VOL 37
15.) Verheyen, A., Diels, A., Dijkmans, J., Oyelami, T., Meneghello, G., Mertens, L., Versweyveld, S., Borgers, M., Buist, A., Peeters, P. & Cik, M. (2015) Using Human iPSC-Derived Neurons to Model TAU Aggregation PLoS One. 10
16.) Sreekanthreddy, P., Gromnicova, R., Davies, H., Phillips, J., Romero, I.A. & Male, D. (2015) A three-dimensional model of the human blood-brain barrier to analyse the transport of nanoparticles and astrocyte/endothelial interactions F1000Res. 4
17.) Rath, B.H., Wahba, A., Camphausen, K. & Tofilon, P.J. (2015) Coculture with astrocytes reduces the radiosensitivity of glioblastoma stem-like cells and identifies additional targets for radiosensitization Cancer Med. 4
18.) Pan, J., Wang, H., Liu, X., Hu, J., Song, W., Luo, J., Jiang, S., Yan, F. & Zhai, B. (2015) Tumor Restrictive Suicide Gene Therapy for Glioma Controlled by the FOS Promoter PLoS One. 10
19.) Lin, J.J., Zhao, T.Z., Cai, W.K., Yang, Y.X., Sun, C., Zhang, Z., Xu, Y.Q., Chang, T. & Li, Z.Y. (2015) Inhibition of histamine receptor 3 suppresses glioblastoma tumor growth, invasion, and epithelial-to-mesenchymal transition Oncotarget. 6
20.) Li, J., Zhou, J., Zhang, D., Song, Y., She, J. & Bai, C. (2015) Bone marrow-derived mesenchymal stem cells enhance autophagy via PI3K/AKT signalling to reduce the severity of ischaemia/reperfusion-induced lung injury J Cell Mol Med. 19
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| MEDIA | |||||
| 1801 | AM | Astrocyte Medium |
$127.00
As low as:
$85.00
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| MOLECULAR BIOLOGY | |||||
| 1804 | HA cDNA | Human Astrocyte cDNA |
$453.00
|
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| 1805 | HA tRNA | Human Astrocyte Total RNA |
$401.00
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| 1806 | HA Lysate | Human Astrocyte Lysate |
$438.00
|
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| 1807 | HA miRNA | Human Astrocyte MicroRNA |
$374.00
|
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| 1809 | HA gDNA | Human Astrocyte genomic DNA |
$438.00
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