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- Human Brain Microvascular Endothelial Cells
Brain microvascular endothelial cells (BMEC), the major component of the blood-brain barrier, limit the passage of soluble and cellular substances from the blood into the brain. BMEC have unique features to distinguish themselves from those of peripheral endothelial cells, such as 1) intercellular tight junctions that display high electrical resistance and slow paracellular flux, 2) the absence of fenestrae and a reduced level of pinocytic activity, and 3) asymmetricallylocalized enzymes and carrier-mediated transport systems. Similar to peripheral endothelial cells, BMEC express, or can be induced to express, cell adhesion molecules on their surface that regulate the extravasation of leukocytes into the brain. BMEC have been widely used for studying the molecular and cellular properties of blood-brain barrier because of their unique
functions.
HBMEC from ScienCell Research Laboratories are isolated from human brain. HBMEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HBMEC are characterized by immunofluorescence with antibodies specific to vWF and/or CD31 (PECAM1). HBMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HBMEC are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, HBMEC are not recommended for expanding or long-term cultures due to limited expansion capacity.
Recommended Medium
It is recommended to use Endothelial Cell Medium (ECM, Cat. #1001) for culturing HBMEC in vitro.
Catalog No. | 1000 |
---|---|
Country of Manufacture | United States |
Product Code | HBMEC |
Size/Quantity | 5 x 10⁵ cells/vial. |
Product Use | HBMEC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures. |
Storage | Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments. |
Shipping Info | Dry ice. |
References | [1] Crone, C. and Oleson, S. P. (1992) Electrical resistance of brain microvessel endothelium. Brain Res. 241: 49-55. [2] Reese, T. S. and Karnovsky, M. J. (1967) Fine structural localization of blood-brain barrier to exogenous peroxidase. J. Cell Biol. 34:9-14. [3] Vorbrodt, A. W. (1988) Ultrastructural cytochemistry of blood-brain barrier endothelia. Prog. Histochem. Cytochem. 18(3):1-96. |
1.) Lu, Y., Chen, X., Liu, X., Shi, Y., Wei, Z., Feng, L., Jiang, Q., Ye, W., Sasaki, T., Fukunaga, K., Ji, Y., Han, F., & Lu, Y.-M. (2023). Endothelial TFEB signaling-mediated autophagic disturbance initiates microglial activation and cognitive dysfunction. Autophagy, 1–18. https://doi.org/10.1080/15548627.2022.2162244
2.) Seo, S., Jang, M., Kim, H., Sung, J. H., Choi, N., Lee, K., & Kim, H. N. (2022). Neuro‐glia‐vascular‐on‐a‐chip system to assess aggravated neurodegeneration via brain endothelial cells upon exposure to diesel exhaust particles. Advanced Functional Materials, 2210123. https://doi.org/10.1002/adfm.202210123
3.) Zhou, Y., Zhang, H., Zheng, B., Ye, L., Zhu, S., Johnson, N.R., Wang, Z., Wei, X., Chen, D., Cao, G., Fu, X., Li, X., Xu, H.Z. & Xiao, J. (2016) Retinoic Acid Induced-Autophagic Flux Inhibits ER-Stress Dependent Apoptosis and Prevents Disruption of Blood-Spinal Cord Barrier after Spinal Cord Injury Int J Biol Sci. 12
4.) Hu, M. & Liu, B.(2016) 'Resveratrol attenuates lipopolysaccharideinduced dysfunction of bloodbrain barrier in endothelial cells via AMPK activation' Korean J Physiol Pharmacol. VOL 20
5.) Wurth, R., Tarn, K., Jernigan, D., Fernandez, S.V., Cristofanilli, M., Fatatis, A. & Meucci, O. (2015) A Preclinical Model of Inflammatory Breast Cancer to Study the Involvement of CXCR4 and ACKR3 in the Metastatic Process Transl Oncol. 8
6.) Liu, N., Li, A.L., Zhou, X.P., Chen, Q. & Cao, W. (2015) P120 catenin attenuates lipopolysaccharide-induced blood-brain barrier dysfunction and inflammatory responses in human brain microvascular endothelial cells Int J Clin Exp Pathol. 8
7.) Li, Y., Zhou, S., Li, J., Sun, Y., Hasimu, H., Liu, R. & Zhang, T. (2015) Quercetin protects human brain microvascular endothelial cells from fibrillar ?-amyloid1?40-induced toxicity Acta Pharm Sin B. 5
8.) Li, J., Zhou, J., Zhang, D., Song, Y., She, J. & Bai, C. (2015) Bone marrow-derived mesenchymal stem cells enhance autophagy via PI3K/AKT signalling to reduce the severity of ischaemia/reperfusion-induced lung injury J Cell Mol Med. 19
9.) Hind, W.H., Tufarelli, C., Neophytou, M., Anderson, S.I., England, T.J. & O'Sullivan, S.E. (2015) Endocannabinoids modulate human blood?brain barrier permeability in vitro Br J Pharmacol. 172
10.) Hempel, C., Boisen, I.M., Efunshile, A., Kurtzhals, J.A. & Staals?, T. (2015) An automated method for determining the cytoadhesion of Plasmodium falciparum-infected erythrocytes to immobilized cells Malar J. 14
11.) Lim PJ, Chu JJH. (2014) "A Polarized Cell Model for Chikungunya Virus Infection: Entry and Egress of Virus Occurs at the Apical Domain of Polarized Cells." PLoSnegl trop dis. 8: e2661.
12.) Pilakka-Kanthikeel S, Atluri VSR, Sagar V, Saxena SK, Nair M. (2013) "Targeted brain derived neurotropic factors (BDNF) delivery across the blood-brain barrier for neuro-protection using magnetic nano carriers: an in-vitro study." PLoS One. 8: e62241.
13.) Cucullo L, Hossain M, Tierney W, Janigro D. (2013) "A new dynamic in vitro modular capillaries-venules modular system:
Cerebrovascular physiology in a box." BMC Neuroscience. 14:18
14.) Muniz-Feliciano L, Van GJ, Portillo JAC, Liew L, Liu B, Carlin CR, Carruthers VB, Matthews S, Subauste CS. (2013) "Toxoplasma gondii-Induced Activation of EGFR Prevents Autophagy Protein-Mediated Killing of the Parasite." PLoSPathog. 9: e1003809.
15.) Seo HS, Minasov G, Seepersaud R, Doran KS, Dubrovska I, Shuvalova L, Wayne AF, Tina IM, Sullam PM. (2013) "Characterization of fibrinogen binding by glycoproteins Srr1 and Srr2 of Streptococcus agalactiae." J Biol Chem. 288: 35982-96.
16.) Claessens A, Adams Y, Ghumra A, Lindergard G, Buchan CC, Andisi C, Bull PC, Mok S, Gupta AP, Wang CW, Turner L, Arman M, Raza A, Bozdech Z, Rowe JA. (2012) "A subset of group A-like var genes encodes the malaria parasite ligands for binding to human brain endothelial cells." Proc Natl Acad Sci USA. 109: E1772-81.
17.) Yan J, Zhang Z, Shi H. (2012) "HIF-1 is involved in high glucose-induced paracellular permeability of brain endothelial cells." Cell Mol Life Sci. 69: 115-28.
18.) Cucullo L, Hossain M, Puvenna V, Marchi N, Janigro D. (2011) "The role of shear stress in Blood-Brain Barrier endothelial physiology." BMC Neurosci. 12: 40.
19.) Feng S, Cen J, Huang Y, Shen H, Yao L, Wang Y, Chen Z. (2011) "Matrix metalloproteinase-2 and -9 secreted by leukemic cells increase the permeability of blood-brain barrier by disrupting tight junction proteins." PLoS One. 6: e20599.
20.) Grau S, Thorsteinsdottir J, von Baumgarten L, Winkler F, Tonn JC, Schichor C. (2011) "Bevacizumab can induce reactivity to VEGF-C and -D in human brain and tumour derived endothelial cells." J Neurooncol. 104: 103-12.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
---|---|---|---|---|---|
MEDIA | |||||
1001 | ECM | Endothelial Cell Medium |
$139.00
As low as:
$92.00
|
||
8248 | BPF | Bovine Plasma Fibronectin |
$137.00
|
||
MOLECULAR BIOLOGY | |||||
1004 | HBMEC cDNA | Human Brain Microvascular Endothelial Cell cDNA |
$453.00
|
||
1005 | HBMEC tRNA | Human Brain Microvascular Endothelial Cell Total RNA |
$401.00
|
||
1006 | HBMEC Lysate | Human Brain Microvascular Endothelial Cell Lysate |
$438.00
|
||
1007 | HBMEC miRNA | Human Brain Microvascular Endothelial Cell MicroRNA |
$374.00
|
||
1009 | HBMEC gDNA | Human Brain Microvascular Endothelial Cell Genomic DNA |
$438.00
|