- Home /
- Human Brain Vascular Pericytes
Pericytes are contractile smooth muscle-like cells that cover the abluminal surface of microvessels. They are most abundant on venules and are common on capillaries. The pericyte population is highly variable between different tissues and organs and appears to show functional heterogeneity. Brain vascular pericytes play a crucial role in the formation and functionality of the blood-brain barrier, regulation of angiogenesis, survival of endothelial cells, and clearance and phagocytosis of cellular debris. Due to the close relationship with endothelial cell structure and blood flow, an excess or deficiency in vascular pericyte activity has been implicated in various pathological conditions including hypertension, diabetic retinopathy, Alzheimer’s disease, multiple sclerosis, and central nervous system tumor formation.
HBVP from ScienCell Research Laboratories are isolated from human brain. HBVP are cryopreserved at passage one after purification and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HBVP are characterized by immunofluorescence with antibody specific to α-smooth muscle actin. HBVP are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HBVP are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Pericyte Medium (PM, Cat. #1201) for culturing HBVP in vitro.
Catalog No. | 1200 |
---|---|
Country of Manufacture | United States |
Product Code | HBVP |
Size/Quantity | 5 x 10^5 cells/vial |
Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. |
Shipping Info | Dry ice. |
References | [1] Sims, D. E. (2000) Diversity within pericytes. Clin. Exp. Pharmacol. Physiol. 27:842-846. [2] Rucker, H. K., Wynder, H. J. and Thomas, W. E. (2000) Cellular mechanisms of CNS pericytes. Brain Res. Bull. 15:363-369. [3] Allt, G. and Lawrenson, J. G. (2001) Pericytes: cell biology and pathology. Cells Tissues Organs. 169:1-11. |
1.) Zhou, Y., Zhang, H., Zheng, B., Ye, L., Zhu, S., Johnson, N.R., Wang, Z., Wei, X., Chen, D., Cao, G., Fu, X., Li, X., Xu, H.Z. & Xiao, J. (2016) Retinoic Acid Induced-Autophagic Flux Inhibits ER-Stress Dependent Apoptosis and Prevents Disruption of Blood-Spinal Cord Barrier after Spinal Cord Injury Int J Biol Sci. 12
2.) Navarro, R., Delgado-Wicke, P., Nunez-Prado, N., Compte, M., Blanco-Toribio, A., Nunez, G., Alvarez-Vallina, L. & Sanz, L. (2016) Role of nucleotide-binding oligomerization domain 1 (NOD1) in pericyte-mediated vascular inflammation J Cell Mol Med. 20
3.) Gurnik, S., Devraj, K., Macas, J., Yamaji, M., Starke, J., Scholz, A., Sommer, K., Di Tacchio, M., Vutukuri, R., Beck, H., Mittelbronn, M., Foerch, C., Pfeilschifter, W., Liebner, S., Peters, K.G., Plate, K.H. & Reiss, Y. (2016) Angiopoietin-2-induced blood-brain barrier compromise and increased stroke size are rescued by VE-PTP-dependent restoration of Tie2 signaling Acta Neuropathol. 131
4.) Yuan, K., Orcholski, M.E., Panaroni, C., Shuffle, E.M., Huang, N.F., Jiang, X., Tian, W., Vladar, E.K., Wang, L., Nicolls, M.R., Wu, J.Y. & de Jesus Perez, V.A. (2015) Activation of the Wnt/Planar Cell Polarity Pathway Is Required for Pericyte Recruitment during Pulmonary Angiogenesis Am J Pathol. 185
5.) Yang, W.J., Hu, J., Uemura, A., Tetzlaff, F., Augustin, H.G. & Fischer, A. (2015) Semaphorin-3C signals through Neuropilin-1 and PlexinD1 receptors to inhibit pathological angiogenesis EMBO Mol Med. 7
6.) Vanlandewijck, M., Lebouvier, T., Andaloussi Mae, M., Nahar, K., Hornemann, S., Kenkel, D., Cunha, S.I., Lennartsson, J., Boss, A., Heldin, C.H., Keller, A. & Betsholtz, C. (2015) Functional Characterization of Germline Mutations in PDGFB and PDGFRB in Primary Familial Brain Calcification PLoS One. 10
7.) Schweller, R.M. & West, J.L. (2015) Encoding Hydrogel Mechanics via Network Cross-Linking Structure ACS Biomater Sci Eng. 1
8.) Li, J., Zhou, J., Zhang, D., Song, Y., She, J. & Bai, C. (2015) Bone marrow-derived mesenchymal stem cells enhance autophagy via PI3K/AKT signalling to reduce the severity of ischaemia/reperfusion-induced lung injury J Cell Mol Med. 19
9.) Bai, Y., Zhu, X., Chao, J., Zhang, Y., Qian, C., Li, P., Liu, D., Han, B., Zhao, L., Zhang, J., Buch, S., Teng, G., Hu, G. & Yao, H. (2015) Pericytes Contribute to the Disruption of the Cerebral Endothelial Barrier via Increasing VEGF Expression: Implications for Stroke PLoS One. 10
10.) You WK, Yotsumoto F, Sakimura K, Adams RH, Stallcup WB. (2014) 'NG2 proteoglycan promotes tumor vascularization via integrin-dependent effects on pericyte function.'
11.) Santos ARC, Dvoriantchikova G, Li Y, Mohammad G, El-Asrar AMA, Wen R, Ivanov D. (2014) 'Cellular Mechanisms of High Mobility Group 1 (HMGB-1) Protein Action in the Diabetic Retinopathy.'
12.) Lippmann ES, Al-Ahmad A, Azarin SM, Palecek SP, Shusta EV. (2014) 'A retinoic acid-enhanced, multicellular human blood-brain barrier model derived from stem cell sources.'
13.) Lippmann ES, Al-Ahmad A, Azarin SM, Palecek SP, Shusta EV. (2014) "A retinoic acid-enhanced, multicellular human blood-brain barrier model derived from stem cell sources." 4.
14.) Santos ARC, Dvoriantchikova G, Li Y, Mohammad G, El-Asrar AMA, Wen R, Ivanov D. (2014) "Cellular Mechanisms of High Mobility Group 1 (HMGB-1) Protein Action in the Diabetic Retinopathy." PLOS ONE. 9: e87574.
15.) You WK, Yotsumoto F, Sakimura K, Adams RH, Stallcup WB. (2014) "NG2 proteoglycan promotes tumor vascularization via integrin-dependent effects on pericyte function." Angiogenesis. 17: 61-76.
16.) Hsu, J., Rappaport, J. & Muro, S. (2014) Specific Binding, Uptake, and Transport of ICAM-1-Targeted Nanocarriers Across Endothelial and Subendothelial Cell Components of the Blood-Brain Barrier Pharm Res. 31
17.) Urich E, Patsch C, Aigner S, Graf M, Iacone R, Freskgrd P. (2013) "Multicellular self-assembled spheroidal model of the blood brain barrier." Sci Rep. 3: 1500.
18.) Mandrup OA, Friis NA, Lykkemark S, Just J, Kristensen P. (2013) "A Novel Heavy Domain Antibody Library with Functionally Optimized Complementarity Determining Regions." PLoS One. 8: e76834.
19.) Glinskii OV, Huxley VH, Glinskii VV, Rubin LJ, Glinsky VV. (2013) 'Pulsed Estrogen Therapy Prevents Post-OVX Porcine Dura Mater Microvascular Network Weakening via a PDGF-BB-Dependent Mechanism.' PloS one.
20.) Glinskii OV, Huxley VH, Glinskii VV, Rubin LJ, Glinsky VV. (2013) "Pulsed Estrogen Therapy Prevents Post-OVX Porcine Dura Mater Microvascular Network Weakening via a PDGF-BB-Dependent Mechanism." PloS one. 8: e82900.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
Publications Promotion
Has your paper been published using one or more of Sciencell products? Get rewarded! Receive 10% off one order. See details >>
CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
---|---|---|---|---|---|
MEDIA | |||||
1201 | PM | Pericyte Medium |
$120.00
As low as:
$81.00
|
||
REAGENTS | |||||
0103 | T/E | Trypsin/EDTA Solution (0.25%; 100ml) |
$21.00
|
||
0113 | TNS | Trypsin Neutralization Solution, 100 ml |
$21.00
|
||
0303 | DPBS | Dulbecco's Phosphate-Buffered Saline, 500 ml |
$16.00
|
||
0403 | PLL | Poly-L-Lysine, 1 mg/ml |
$16.00
|
||
MOLECULAR BIOLOGY | |||||
1204 | HBVP cDNA | Human Brain Vascular Pericyte cDNA |
$411.00
|
||
1205 | HBVP tRNA | Human Brain Vascular Pericyte Total RNA |
$364.00
|
||
1206 | HBVP Lysate | Human Brain Vascular Pericyte Lysate |
$398.00
|
||
1207 | HBVP miRNA | Human Brain Vascular Pericyte MicroRNA |
$340.00
|
||
1209 | HBVP gDNA | Human Brain Vascular Pericytes genomic DNA |
$398.00
|