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- Human Bronchial Epithelial Cells
The respiratory epithelium is composed of a mixed population of ciliated, nonciliated, and mucoussecreting cells from proximal to distal airways. The individual characteristics of these cells create not only an effective physical barrier against various noxious substances, but also a highly sophisticated host defense system by producing and releasing a large number of chemical mediators and cytokines. The bronchial epithelium consists of the surface epithelial cells and mucus glands. The surface epithelial cells are made up of three principle cell types: basal, goblet, and ciliated cells, of which the latter two form a suprabasal columnar structure and are necessary for mucociliary clearance. Studies using bronchial epithelial cells (BEpiC) have demonstrated that IL-4 and IL-13 stimulation can modify cellular proliferation, ciliary beating, and mucous production. BEpiC proliferation is also regulated in part by EGF receptor signaling. Cultured BEpiC are a useful in vitro model for studying the function and pathophysiology of the bronchial epithelium.
HBEpiC from ScienCell Research Laboratories are isolated from human bronchi. HBEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HBEpiC are characterized by immunofluorescence with antibodies specific to cytokeratin18 and/or cytokeratin-19. HBEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HBEpiC are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Bronchial Epithelial Cell Medium (BEpiCM, Cat. #3211) for culturing HBEpiC in vitro.
Catalog No. | 3210 |
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Country of Manufacture | United States |
Product Code | HBEpiC |
Size/Quantity | 5 x 10^5 cells/vial |
Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. |
Shipping Info | Dry ice. |
References | [1]. Velden, V. H., and H. F. Versnel. 1998. Bronchial epithelium: morphology, function and pathophysiology in asthma. Eur. Cytokine Netw. 9:585-597. [2]. Kikuchi, T., Shively, J.D., Foley, J.S., Drazen, J.M., Tschumperlin, D.J. (2004) Differentiation-dependent responsiveness of bronchial epithelial cells to IL-4/13 stimulation. Am J Physiol Lung Cell Mol Physiol. 287:L119-26. [3]. Kim S, Schein AJ, Nadel JA.(2005) E-cadherin promotes EGFR-mediated cell differentiation and MUC5AC mucin expression in cultured human airway epithelial cells. Am J Physiol Lung Cell Mol Physiol. 289:L1049-60. |
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ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
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MEDIA | |||||
3211 | BEpiCM | Bronchial Epithelial Cell Medium |
$135.00
As low as:
$90.00
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MOLECULAR BIOLOGY | |||||
3214 | HBEpiC cDNA | Human Bronchial Epithelial Cell cDNA |
$453.00
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3215 | HBEpiC tRNA | Human Bronchial Epithelial Cell Total RNA |
$401.00
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3216 | HBEpiC Lysate | Human Bronchial Epithelial Cell Lysate |
$438.00
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3217 | HBEpiC miRNA | Human Bronchial Epithelial Cell MicroRNA |
$374.00
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3219 | HBEpiC gDNA | Human Bronchial Epithelial Cell genomic DNA |
$438.00
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