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How can we help you today?
Need help with product selection and ordering?
Email us at sales@sciencellonline.com
Need help with scientific inquiries, technical assistance, product applications, and use?
Email us at techsupport@sciencellonline.com
Need help with issues concerning this website?
Email us at webmaster@sciencellonline.com
Have any marketing related inquiries?
Email us at marketing@sciencellonline.com
Need help ordering from abroad?
As the largest cell provider, we are available worldwide.
Please visit our Distributors page to select the company nearest to your location.
We are located at 1610 Faraday Ave, Carlsbad, CA 92008
Call us at (877) 602-8549 or (760) 602-8549
Fax us at (760) 602-8575
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ScienCell’s OneStep Human CA19-9 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CA19-9 with a 96-well strip plate that is pre-coated with antibody specific for CA19-9. The detection antibody is a HRP conjugated antibody specific for CA19-9. The capture antibody is monoclonal antibody from mouse, and the detection antibody is monoclonal antibody from mouse. The kit is analytically validated with ready to use reagents. To measure Human CA19-9, add standards and samples to the wells, then add the HRP conjugated detection antibody. Wash away the unbounded protein and HRP conjugated detection antibody. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. Upon addition of the substrate, the density of the yellow product is linearly proportional to Human CA19-9 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CA19-9 in the sample.
Materials Required But Not Provided
1. Microplate reader.
2. Automated plate washer.
3. Adjustable pipettes and pipette tips. Multi-channel pipettes are recommended for large amount of samples.
4. Clean tubes and Eppendorf tubes.
5. Deionized or distilled water.
6. 500 mL graduated cylinders
Assay type
Sandwich ELISA
Range
10 – 150 U/ml
Sensitivity
3.8 U/ml
Specificity
Natural and recombinant human CA19-9
Cross-reactivity
This kit is for the quantitation of Human CA19-9 concentrations in Serum. No significant cross-reactivity or interference was observed. This claim is limited by existing techniques therefore cross-reactivity may exist with untested analogs.
Application
For quantitative detection of human CA19-9 concentrations in Serum.
Catalog No. | EK7028 |
---|---|
Country of Manufacture | China |
Product Code | CA19-9 OneStep-ELISA |
Size/Quantity | 96 Tests in 8 x 12 divisible strips |
Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
Storage | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles. |
Shipping Info | Shipped with gel ice. |
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