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Cardiac fibroblasts (CF) provide structural support for cardiac myocytes and are responsible for extracellular matrix synthesis in the heart during growth and pathophysiological conditions. CF are an important cellular component of myocardial responses to injury and the source of paracrine growth factors. CF proliferation and synthesis of matrix is essential for scar formation at sites of myocardial infarction and cardiac fibrosis. CF have also been found to respond to mechanical loading to regulate the metabolic activity of ventricular cells, making them a good model to study the cardiac matrix remodeling using physiological (exercise) and pathological (hypertension) stressors.
HCF-av from ScienCell Research Laboratories are isolated from human ventricle. HCF-av are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HCF-av are characterized by immunofluorescence with antibody specific to fibronectin. HCF-av are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HCF-av are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium-2 (FM-2, Cat. #2331) for culturing HCF-av in vitro.
| Catalog No. | 6310 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HCF-av |
| Size/Quantity | 5 x 10^5 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Sabri, A., Short, J., Guo, J., Steinberg, S. F. (2002) Protease-activated receptor-1-mediated DNA synthesis in cardiac fibroblast is via epidermal growth factor receptor transactivation: distinct PAR-1 signaling pathways in cardiac fibroblasts and cardiomyocytes. Circ. Res. 91:532-9. [2] Akiyama-Uchida, Y. et al., (2002) Norepinephrine enhances fibrosis mediated by TGF-beta in cardiac fibroblasts. Hypertension 40:148-54. [3] Burgess, M. L., Terracio, L., Hirozane, T., Borg, T. K. (2002) Differential integrin expression by cardiac fibroblasts from hypertensive and exercise-trained rat hearts. Cardiovasc Pathol 11(2):78-87. |
63101.) Bae, H., Lee, D., Kim, Y.W., Choi, J., Lee, H.J., Kim, S.W., Kim, T., Noh, Y.H., Ko, J.H., Bang, H. & Lim, I. (2016) Effects of hydrogen peroxide on voltage-dependent K+ currents in human cardiac fibroblasts through protein kinase pathways Korean J Physiol Pharmacol. 20
2.) Meredith, A., Boroomand, S., Carthy, J., Luo, Z. & McManus, B. (2015) 1,25 Dihydroxyvitamin D3 Inhibits TGF?1-Mediated Primary Human Cardiac Myofibroblast Activation PLoS One. 10
3.) Li, J., Zhou, J., Zhang, D., Song, Y., She, J. & Bai, C. (2015) Bone marrow-derived mesenchymal stem cells enhance autophagy via PI3K/AKT signalling to reduce the severity of ischaemia/reperfusion-induced lung injury J Cell Mol Med. 19
4.) Watson CJ, Ledwidge MT, Phelan D, Collier P, Byrne JC, Dunn MJ, McDonald KM, Baugh JA. (2011) "Proteomic Analysis of Coronary Sinus Serum Reveals Leucine-Rich {alpha}2-Glycoprotein as a Novel Biomarker of Ventricular Dysfunction and Heart Failure." Circ Heart Fail. 4: 188-97.
5.) Chen JB, Tao R, Sun HY, Tse HF, Lau CP, Li GR. (2010) "Multiple Ca2+ signaling pathways regulate intracellular Ca2+ activity in human cardiac fibroblasts." J Cell Physiol. 223: 68-75.
6.) Li GR, Sun HY, Chen JB, Zhou Y, Tse HF, Lau CP. (2009) "Characterization of multiple ion channels in cultured human cardiac fibroblasts." PLoS One. 4: e7307.
7.) Bunda S, Liu P, Wang Y, Liu K, Hinek A. (2007) "Aldosterone induces elastin production in cardiac fibroblasts through activation of insulin-like growth factor-I receptors in a mineralocorticoid receptor-independent manner." Am J Pathol. 171: 809-19.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| 6314 | HCF-av cDNA | Human Cardiac Fibroblast-adult ventrical cDNA |
$453.00
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| 6315 | HCF-av tRNA | Human Cardiac Fibroblast-adult ventricular Total RNA |
$401.00
|
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| 6316 | HCF-av Lysate | Human Cardiac Fibroblast-adult ventrical Lysate |
$438.00
|
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| 6317 | HCF-av miRNa | Human Cardiac Fibroblast-adult ventrical MicroRNA |
$374.00
|
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| 6319 | HCF-av gDNA | Human Cardiac Fibroblast-adult ventricular Genomic DNA |
$438.00
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