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- Human Cardiac Fibroblasts
Cardiac fibroblasts (CF) provide structural support for cardiac myocytes and are responsible for extracellular matrix synthesis in the heart during growth and pathophysiological conditions. CF are an important cellular component of myocardial responses to injury and the source of paracrine growth factors. CF proliferation and synthesis of matrix is essential for scar formation at sites of myocardial infarction and cardiac fibrosis. CF cultures have been widely used as a model to study the cardiac matrix remodeling by physiological (exercise) and pathological (hypertension) stressors.
HCF from ScienCell Research Laboratories are isolated from human heart. HCF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HCF are characterized by immunofluorescence with antibody specific to fibronectin. HCF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HCF are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium-2 (FM-2, Cat. #2331) for culturing HCF in vitro.
| Catalog No. | 6300 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HCF |
| Size/Quantity | 5 x 10^5 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Sabri, A., Short, J., Guo, J., Steinberg, S. F. (2002) Protease-activated receptor-1-mediated DNA synthesis in cardiac fibroblast is via epidermal growth factor receptor transactivation: distinct PAR-1 signaling pathways in cardiac fibroblasts and cardiomyocytes. Circ. Res. 91:532-9. [2] Akiyama-Uchida, Y. et al., (2002) Norepinephrine enhances fibrosis mediated by TGF-beta in cardiac fibroblasts. Hypertension 40:148-54. [3] Burgess, M. L., Terracio, L, Hirozane, T., Borg, T. K. (2002) Differential integrin expression by cardiac fibroblasts from hypertensive and exercise-trained rat hearts. Cardiovasc Pathol 11(2):78-87. |
63001.) Chida J, Araki H, Maeda Y. (2014) "Specific growth suppression of human cancer cells by targeted delivery of Dictyostelium mitochondrial ribosomal protein S4." Cancer cell int. 14: 56.
2.) Huntley BK, Ichiki T, Sangaralingham SJ, Chen HH, Burnett JC Jr. (2010) "B-type natriuretic peptide and extracellular matrix protein interactions in human cardiac fibroblasts." J Cell Physiol. 225: 251-5.
3.) Lin CS, Pan CH, Wen CH, Yang TH, Kuan TC. (2010) "Regulation of angiotensin converting enzyme II by angiotensin peptides in human cardiofibroblasts." Peptides. 31: 1334-40.
4.) Westenbrink BD, Ruifrok WP, Voors AA, Tilton RG, van Veldhuisen DJ, Schoemaker RG, van Gilst WH, de Boer RA. (2010) "Vascular endothelial growth factor is crucial for erythropoietin-induced improvement of cardiac function in heart failure." Cardiovasc Res. 87: 30-9.
5.) Huntley BK, Ichiki T, Sangaralingham SJ, Chen HH, Burnett JC. (2010) "B?type natriuretic peptide and extracellular matrix protein interactions in human cardiac fibroblasts." J of cell physiol. 225: 251-55.
6.) McKie PM, Cataliotti A, Huntley BK, Martin FL, Olson TM, Burnett JC Jr. (2009) "A human atrial natriuretic peptide gene mutation reveals a novel peptide with enhanced blood pressure-lowering, renal-enhancing, and aldosterone-suppressing actions." J Am Coll Cardiol. 54: 1024-32.
7.) Lisy O, Huntley BK, McCormick DJ, Kurlansky PA, Burnett JC Jr. (2008) "Design, synthesis, and actions of a novel chimeric natriuretic peptide: CD-NP." J Am Coll Cardiol. 52: 60-8.
8.) Pan CH, Wen CH, Lin CS. (2008) "Interplay of angiotensin II and angiotensin(1-7) in the regulation of matrix metalloproteinases of human cardiocytes." Exp Physiol. 93: 599-612.
9.) Reddy VS, Harskamp RE, van Ginkel MW, Calhoon J, Baisden CE, Kim IS, Valente AJ, Chandrasekar B. (2008) "Interleukin-18 stimulates fibronectin expression in primary human cardiac fibroblasts via PI3K-Akt-dependent NF-kappaB activation." J Cell Physiol. 215: 697-707.
10.) Saxena A, Fish JE, White MD, Yu S, Smyth JW, Shaw RM, DiMaio JM, Srivastava D. (2008) "Stromal cell-derived factor-1alpha is cardioprotective after myocardial infarction." Circulation. 117: 2224-31.
11.) Bunda S, Liu P, Wang Y, Liu K, Hinek A. (2007) "Aldosterone induces elastin production in cardiac fibroblasts through activation of insulin-like growth factor-I receptors in a mineralocorticoid receptor-independent manner." Am J Pathol. 171: 809-19.
12.) Cortez DM, Feldman MD, Mummidi S, Valente AJ, Steffensen B, Vincenti M, Barnes JL, Chandrasekar B. (2007) "IL-17 stimulates MMP-1 expression in primary human cardiac fibroblasts via p38 MAPK- and ERK1/2-dependent C/EBP-beta , NF-kappaB, and AP-1 activation." Am J Physiol Heart Circ Physiol. 293: H3356-65.
13.) Noser JA, Mael AA, Sakuma R, Ohmine S, Marcato P, Lee PW, Ikeda Y. (2007) "The RAS/Raf1/MEK/ERK Signaling Pathway Facilitates VSV-mediated Oncolysis: Impli cation for the Defective Interferon Response in Cancer Cells." Mol Ther. 15: 1531-6.
14.) Huntley BK, Sandberg SM, Noser JA, Cataliotti A, Redfield MM, Matsuda Y, Burnett JC Jr. (2006) "BNP-induced activation of cGMP in human cardiac fibroblasts: Interactions with fibronectin and natriuretic peptide receptors." J Cell Physiol. 209: 943-9.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| 6304 | HCF cDNA | Human Cardiac Fibroblast cDNA |
$453.00
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| 6305 | HCF tRNA | Human Cardiac Fibroblast Total RNA |
$401.00
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| 6306 | HCF Lysate | Human Cardiac Fibroblast Lysate |
$438.00
|
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| 6307 | HCF miRNA | Human Cardiac Fibroblast MicroRNA |
$374.00
|
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| 6309 | HCF gDNA | Human Cardiac Fibroblast genomic DNA |
$438.00
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