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- Human Cardiac Myocytes
Cardiac myocytes are the most physically energetic cells in the body. They are highly specialized high-oxygen-content cells that house a large number of mitochondria [1]. They occupy as much as 75% of the cardiac mass, but constitute only about one third of the total cell number in the heart. Differentiated cardiac myocytes have little capacity to proliferate; however, hypertrophic growth has been shown to respond to alpha1-adrenergic stimuli via the Ras/MEK pathway [2]. All cardiac myocytes are capable of spontaneous rhythmic depolarization and repolarization of their membranes. Contraction of cardiac myocytes is myogenic, which is independent of nervous stimulation. There is a complex network of signals in cardiac myocytes regulating the rhythmic pumping of the heart [3]. Cardiac myocyte hypertrophy and apoptosis have been implicated in the loss of contractile function during heart failure. A better understanding of the cardiac signaling network will help reveal the cellular mechanisms regulating to cardiac myocyte death.
HCM from ScienCell Research Laboratories are isolated from human heart. HCM are cryopreserved after purification and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. HCM are characterized by immunofluorescence with antibody specific to sarcomeric α-actinin. HCM are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HCM are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, HCM are not recommended for expanding or long-term cultures since the cells do not proliferate in culture.
Recommended Medium
It is recommended to use Cardiac Myocyte Medium-serum free (CMM-sf, Cat. #6101) for culturing HCM in vitro.
| Catalog No. | 6200 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HCM |
| Size/Quantity | 1 x 10^6 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Bodyak, N., Kang, P. M., Hiromura, M., Sulijoadikusumo, I., Horikoshi, N., Khrapko, K. and Usheva, A. (2002) Gene expression profiling of the aging mouse cardiac myocytes. Nucleic Acids Research 30(17):3788-3794. [2] Tamamori-Adachi, M., Ito, H., Nobori, K., Hayashida, K., Kawauchi, J., Adachi, S., Ikeda, M. A. and Kitajima, S. (2002) Expression of cyclin D1 and CDK4 causes hypertrophic growth of cardiomyocytes in culture: a possible implication for cardiac hypertrophy. Biochem Biophys Res Commun 296(2):274-80. [3] Sambrano, G.R., Fraser, I., Han, H., Ni, Y., O'Connell, T., Yan, Z. and Stull, J. T. (2002) Navigating the signaling network in mouse cardiac myocytes. Nature 420(6916):712-4. |
62001.) Kuo, H.F., Liu, P.L., Chong, I.W., Liu, Y.P., Chen, Y.H., Ku, P.M., Li, C.Y., Chen, H.H., Chiang, H.C., Wang, C.L., Chen, H.J., Chen, Y.C. & Hsieh, C.C.(2016) 'Pigment EpitheliumDerived Factor Mediates Autophagy and Apoptosis in Myocardial Hypoxia/Reoxygenation Injury' PLoS One. VOL 11
2.) Wang E, Sun S, Qiao B, Duan W, Huang G, An Y, Xu S, Zheng Y, Su Z, Gu X, Jin L, Wang H. (2013) "Identification of functional mutations in GATA4 in patients with congenital heart disease." PLoS One. 8: e62138.
3.) Bauwens CL, Song H, Thavandiran N, Ungrin M, Mass? S, Nanthakumar K, Seguin C, Zandstra PW. (2011) "Geometric Control of Cardiomyogenic Induction in Human Pluripotent Stem Cells." Tissue Eng Part A. 17: 1901-9.
4.) Mukhopadhyay P, Rajesh M, B?tkai S, Patel V, Kashiwaya Y, Liaudet L, Evgenov OV, Mackie K, Hask? G, Pacher P. (2010) "CB1 cannabinoid receptors promote oxidative stress and cell death in murine models of doxorubicin-induced cardiomyopathy and in human cardiomyocytes." Cardiovasc Res. 85: 773-84.
5.) Voigt A, Trimpert C, Bartel K, Egerer K, Kuckelkorn U, Feist E, Gericke C, Klingel K, Kandolf R, Felix SB, Baumann G, Kloetzel PM, Stangl K, Staudt A. (2010) "Lack of evidence for a pathogenic role of proteasome-directed autoimmunity in dilated cardiomyopathy." Basic Res Cardiol. 105: 557-67.
6.) Yeh YC, Wei HJ, Lee WY, Yu CL, Chang Y, Hsu LW, Chung MF, Tsai MS, Hwang SM, Sung HW. (2010) "Cellular cardiomyoplasty with human amniotic fluid stem cells: in vitro and in vivo studies." Tissue Eng Part A. 16: 1925-36.
7.) Hussein S, Michael P, Brabant D, Omri A, Narain R, Passi K, Ramana CV, Parrillo JE, Kumar A, Parissenti A, Kumar A. (2009) "Characterization of human septic sera induced gene expression modulation in human myocytes." Int J Clin Exp Med. 2: 131-48.
8.) Kim KS, Chapman NM, Tracy S. (2008) "Replication of coxsackievirus B3 in primary cell cultures generates novel viral genome deletions." J Virol. 82: 2033-7.
9.) Pan CH, Wen CH, Lin CS. (2008) "Interplay of angiotensin II and angiotensin(1-7) in the regulation of matrix metalloproteinases of human cardiocytes." Exp Physiol. 93: 599-612.
10.) Jiang Y, Reynolds C, Xiao C, Feng W, Zhou Z, Rodriguez W, Tyagi SC, Eaton JW, Saari JT, Kang YJ. (2007) "Dietary copper supplementation reverses hypertrophic cardiomyopathy induced by chronic pressure overload in mice." J Exp Med. 204: 657-66.
11.) Li SY, Sigmon VK, Babcock SA, Ren J. (2007) "Advanced glycation endproduct induces ROS accumulation, apoptosis, MAP kinase activation and nuclear O-GlcNAcylation in human cardiac myocytes." Life Sci. 80: 1051-6.
12.) Ng ES, Cheng ZJ, Ellis A, Ding H, Jiang Y, Li Y, Hollenberg MD, Triggle CR. (2007) "Nitrosothiol stores in vascular tissue: Modulation by ultraviolet light, acetylcholine and ionomycin." Eur J Pharmacol. 560: 183-92.
13.) Spector NL, Yarden Y, Smith B, Lyass L, Trusk P, Pry K, Hill JE, Xia W, Seger R, Bacus SS. (2007) "Activation of AMP-activated protein kinase by human EGF receptor 2/EGF receptor tyrosine kinase inhibitor protects cardiac cells." Proc Natl Acad Sci USA. 104: 10607-12.
14.) Li SY, Li Q, Shen JJ, Dong F, Sigmon VK, Liu Y, Ren J. (2006) "Attenuation of acetaldehyde-induced cell injury by overexpression of aldehyde dehydrogenase-2 (ALDH2) transgene in human cardiac myocytes: role of MAP kinase signaling." J Mol Cell Cardiol. 40: 283-94.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| MEDIA | |||||
| 6101 | CMM-sf | Cardiac Myocyte Medium-serum free |
$137.00
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| 6201 | CMM | Cardiac Myocyte Medium |
$137.00
As low as:
$90.00
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| MOLECULAR BIOLOGY | |||||
| 6204 | HCM cDNA | Human Cardiac Myocyte cDNA |
$453.00
|
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| 6205 | HCM tRNA | Human Cardiac Myocyte Total RNA |
$401.00
|
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| 6206 | HCM Lysate | Human Cardiac Myocyte Lysate |
$438.00
|
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| 6207 | HCM miRNA | Human Cardiac Myocyte MicroRNA |
$374.00
|
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| 6209 | HCM gDNA | Human Cardiac Myocyte genomic DNA |
$438.00
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