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- Human Colonic Epithelial Cells
The colorectum is a major organ in both malignant and nonmalignant diseases. Cells that line the colonic mucosal surface form a major mechanical barrier that separates the host’s internal milieu from the external environment. In addition to the well-established role of epithelial cells in ion transport, these cells appear to function as an integral component of the mucosal immune system. Human colonic epithelial cells (HCoEpiC) can process and present antigens to T cells in vitro, and can be stimulated to express HLA class II and intercellular adhesion molecules in vivo. They also respond to a broad array of cytokines with altered gene expression and growth characteristics. In addition, normal colonic epithelial cells were found to express the α3, α5, α6, β1 and β4 integrin.
HCoEpiC from ScienCell Research Laboratories are isolated from human colonic tissue. HCoEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HCoEpiC are characterized by immunofluorescence with antibodies specific to CK18 and CK19. HCoEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCoEpiC are guaranteed to further culture for 10 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Colonic Epithelial Cell Medium (CoEpiCM, Cat. #2951) for culturing HCoEpiC in vitro.
| Catalog No. | 2950 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HCoEpiC |
| Size/Quantity | 5 x 10^5 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Mayer, L., Eisenhardt, D., Salomon, P., Bauer, W., Plous, R. and Piccinini, L. (1991) Expression of class II molecules on intestinal epithelial cells in humans. Differences between normal and inflammatory bowel disease. Gastroenterology. 100:3-12. [2] Eckmann, L., Jung H.-C., Schuerer-Maly, C.-C., Panja, A., Morzycka-Wroblewska, E., and Kagnoff, M. F. (1993) Differential cytokine expression by human intestinal epithelial cell lines: regulated expression of interleukin-8. Gastroenterology. 105:1689-1697. [3] Stallmach, A., v Lampe, B., Matthes, H., Bornhoft, B., and Riecken, E. O. (1992) Diminished expression of integrin adhesion molecules on human colonic epithelial cells during the benign to malign tumor. Gut 33:342-346. |
29501.) Das, U.N. (2016) Inflammatory bowel disease as a disorder of an imbalance between pro- and anti-inflammatory molecules and deficiency of resolution bioactive lipids Lipids Health Dis. 15
2.) Richards, A.L., Burns, M.B., Alazizi, A., Barreiro, L.B., PiqueRegi, R., Blekhman, R. & Luca, F.(2016) 'Genetic and transcriptional analysis of human host response to healthy gut microbiota' mSystems. VOL 1
3.) Tsai, C.C., Chuang, T.W., Chen, L.J., Niu, H.S., Chung, K.M., Cheng, J.T. & Lin, K.C. (2015) Increase in apoptosis by combination of metformin with silibinin in human colorectal cancer cells World J Gastroenterol. 21
4.) Li, J., Zhou, J., Zhang, D., Song, Y., She, J. & Bai, C. (2015) Bone marrow-derived mesenchymal stem cells enhance autophagy via PI3K/AKT signalling to reduce the severity of ischaemia/reperfusion-induced lung injury J Cell Mol Med. 19
5.) Hua H, Chen W, Shen L, Sheng Q, Teng L. (2013) 'Honokiol augments the anti-cancer effects of oxaliplatin in colon cancer cells.'
6.) Rabineau M, Kocgozlu L, Dujardin D, Senger B, Haikel Y, Voegel JC, Freund JN, Schaaf P, Lavalle P, Vautier D. (2013) 'Contribution of Soft Substrates to Malignancy and Tumor Suppression during Colon Cancer Cell Division.'
7.) Hua H, Chen W, Shen L, Sheng Q, Teng L. (2013) "Honokiol augments the anti-cancer effects of oxaliplatin in colon cancer cells." ActaBiochimBiophys Sin.45: 773-79.
8.) Rabineau M, Kocgozlu L, Dujardin D, Senger B, Haikel Y, Voegel JC, Freund JN, Schaaf P, Lavalle P, Vautier D. (2013) "Contribution of Soft Substrates to Malignancy and Tumor Suppression during Colon Cancer Cell Division." PloS One. 8: e78468.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
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| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| MEDIA | |||||
| 2951 | CoEpiCM | Colonic Epithelial Cell Medium |
$120.00
As low as:
$78.00
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| REAGENTS | |||||
| 0103 | T/E | Trypsin/EDTA Solution, 0.25% |
$21.00
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| 0113 | TNS | Trypsin Neutralization Solution, 100 ml |
$21.00
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| 0303 | DPBS | Dulbecco's Phosphate-Buffered Saline, 500 ml |
$16.00
|
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| 0403 | PLL | Poly-L-Lysine, 1 mg/ml |
$16.00
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| 0973 | EpiF | EpiFectagen II |
$253.00
|
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| MOLECULAR BIOLOGY | |||||
| 2954 | HCoEpiC cDNA | Human Colonic Epithelial Cell cDNA |
$411.00
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| 2955 | HCoEpiC tRNA | Human Colonic Epithelial Cell Total RNA |
$364.00
|
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| 2956 | HCoEpiC Lysate | Human Colonic Epithelial Cell Lysate |
$398.00
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| 2959 | HCEpiC gDNA | Human Colonic Epithelial Cell Genomic DNA |
$398.00
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| 2957 | HCoEpiC miRNA | Human Colonic Epithelial Cell MicroRNA |
$340.00
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