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- Human Colonic Smooth Muscle Cells
Smooth muscle contraction is the fundamental event in gastrointestinal motion. Inflammation of the human intestine causes increased levels of smooth muscle-specific actin, which in turn promotes the thickening of the smooth muscle layers. The increased smooth muscle actin may affect force production and further demonstrates the plasticity of smooth muscle cells in the inflamed intestine. Studies also show that human intestinal smooth muscle cells respond to IL-1beta and TNFalpha stimulation by releasing IL-6, which may significantly contribute to the overall systemic inflammatory response. The colonic smooth muscle cells (CoSMC) possess a different subtype of somatostatin receptor from gastric smooth muscle cells. The availability of human CoSMC makes it more feasible to study the contractile and proliferative responses of human colonic smooth muscle.
HCoSMC from ScienCell Research Laboratories are isolated from the human colon. HCoSMC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HCoSMC are characterized by immunofluorescence with antibodies specific to α-smooth muscle actin. HCoSMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCoSMC are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Smooth Muscle Cell Medium (SMCM, Cat. #1101) for culturing HCoSMC in vitro.
Catalog No. | 2940 |
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Country of Manufacture | United States |
Product Code | HCoSMC |
Size/Quantity | 5 x 10⁵ cells/vial. |
Product Use | HCoSMC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures. |
Storage | Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments. |
Shipping Info | Dry ice. |
References | [1]. Blennerhassett, M. G., Bovell, F. M., Lourenssen, S., McHugh, K. M. (1999) Characteristics of inflammation-induced hypertrophy of rat intestinal smooth muscle cell. Dig Dis Sci. 44(7):1265-72. [2]. Ng, E. K., Panesar, N., Longo, W. E., Shapiro, M. J., Kaminski, D. L., Tolman, K. C., Mazuski, J. E. (2003) Human intestinal smooth muscle cells are potent producers of IL-6. Mediators Inflamm. 12(1):3-8. [3] Corleto, V.D., Severi, C., Coy, D.H., Delle, Fave G., Jensen, R.T. (1997) Colonic smooth muscle cells possess a different subtype of somatostatin receptor from gastric smooth muscle cells. Am J Physiol. 272:G689-97. |
1.) Zhu QS, Rosenblatt K, Huang KL, Lahat G, Brobey R, Bolshakov S, Nguyen T, Ding Z, Belousov R, Bill K, Luo X, Lazar A, Dicker A, Mills GB, Hung MC, Lev D. (2011) "Vimentin is a novel AKT1 target mediating motility and invasion." Oncogene. 30: 457-70.
2.) Cen L, Hutzen B, Ball S, DeAngelis S, Chen CL, Fuchs JR, Li C, Li PK, Lin J. (2009) "New structural analogues of curcumin exhibit potent growth suppressive activity in human colorectal carcinoma cells." BMC Cancer. 9: 99.
3.) Wei YC, Li CF, Yu SC, Chou FF, Fang FM, Eng HL, Uen YH, Tian YF, Wu JM, Li SH, Huang WW, Li WM, Huang HY. (2009) "Ezrin overexpression in gastrointestinal stromal tumors: an independent adverse prognosticator associated with the non-gastric location." Mod Pathol. 22: 1351-60.
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CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
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MEDIA | |||||
1101 | SMCM | Smooth Muscle Cell Medium |
$114.00
As low as:
$78.00
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REAGENTS | |||||
0103 | T/E | Trypsin/EDTA Solution (0.25%; 100ml) |
$21.00
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0113 | TNS | Trypsin Neutralization Solution, 100 ml |
$21.00
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0303 | DPBS | Dulbecco's Phosphate-Buffered Saline, 500 ml |
$16.00
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0403 | PLL | Poly-L-Lysine, 1 mg/ml |
$16.00
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0953 | SMCF | SMCFectagen |
$253.00
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MOLECULAR BIOLOGY | |||||
2944 | HCoSMC cDNA | Human Colonic Smooth Muscle Cell cDNA |
$411.00
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2945 | HCoSMC tRNA | Human Colonic Smooth Muscle Cell Total RNA |
$364.00
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2946 | HCoSMC Lysate | Human Colonic Smooth Muscle Cell Lysate |
$398.00
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2947 | HCoSMC miRNA | Human Colonic Smooth Muscle Cell MicroRNA |
$340.00
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2949 | HCSMC gDNA | Human Colonic Smooth Muscle Cell genomic DNA |
$398.00
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