- Home /
- Human Conjunctival Fibroblasts
Conjunctival fibroblasts, like other fibroblasts, are responsible for fibrosis, matrix production, and tissue remodeling. They are also actively involved as an effector in ocular allergies. In chronic allergic conjunctivitis, conjunctival fibroblasts responded to histamine to increase proliferation and induce the production of proinflammatory cytokines, such as IL-1, IL-6, and IL-8, to perpetuate allergic inflammation. Conjunctival fibroblast cultures can be used as an in vitro model for rapid screening of drugs and may provide a basis for the development of new treatments for ocular proliferative disorders.
HConF from ScienCell Research Laboratories are isolated from human conjunctiva. HConF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HConF are characterized by their spindle morphology and immunofluorescence with antibody specific to fibronectin. HConF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HConF are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing HConF in vitro.
| Catalog No. | 6570 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HConF |
| Size/Quantity | 5 x 10^5 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Asano-Kato, N., Fukagawa, K., Okada, N., Dogru, M., Tsubota, K., Fujishima, H. (2005) Tryptase increases proliferative activity of human conjunctival fibroblasts through protease-activated receptor-2. Invest Ophthalmol Vis Sci. 46(12):4622-6. [2] Solomon, A., Puxeddu, I., Levi-Schaffer, F.(2003) Fibrosis in ocular allergic inflammation: recent concepts in the pathogenesis of ocular allergy. Curr Opin Allergy Clin Immunol 3(5):389-393. |
65701.) Ninomiya, I., Yamatoya, K., Mashimo, K., Matsuda, A., Usui-Ouchi, A., Araki, Y., & Ebihara, N. (2022). Role of oncostatin M in the pathogenesis of vernal keratoconjunctivitis: Focus on the barrier function of the epithelium and interleukin-33 production by fibroblasts. Investigative Opthalmology & Visual Science, 63(13), 26. https://doi.org/10.1167/iovs.63.13.26
2.) Cavet ME, Volhejn S, Harrington KL, Zhang JZ. (2013) "Anti-allergic effects of mapracorat, a novel selective glucocorticoid receptor agonist, in human conjunctival fibroblasts and epithelial cells." Mol Vis. 19: 1515-25.
3.) Zhang JZ, Cavet ME, VanderMeid KR, Salvador-Silva M, L?pez FJ, Ward KW. (2009) "BOL-303242-X, a novel selective glucocorticoid receptor agonist, with full anti-inflammatory properties in human ocular cells." Mol Vis. 15: 2606-16.
4.) Swaney JS, Moreno KM, Gentile AM, Sabbadini RA, Stoller GL. (2008) "Sphingosine-1-phosphate (S1P) is a novel fibrotic mediator in the eye." Exp Eye Res. 87: 367-75.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| 6574 | HConF cDNA | Human Conjunctival Fibroblast cDNA |
$453.00
|
||
| 6575 | HConF tRNA | Human Conjunctival Fibroblast Total RNA |
$401.00
|
||
| 6576 | HConF Lysate | Human Conjunctival Fibroblast Lysate |
$438.00
|
||
| 6577 | HConF miRNA | Human Conjunctival Fibroblast MicroRNA |
$374.00
|
||
| 6579 | HConF gDNA | Human Conjunctival Fibroblast Genomic DNA |
$438.00
|
