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- Human Coronary Artery Endothelial Cells
The coronary arteries provide a substantial blood supply to meet the enormous energy demands of the heart for its constant contractile activity. Lining the vessel wall, coronary artery endothelial cells (CAEC) are continuously exposed to fluid shear stress that induces changes in cell morphology and the production of endothelium-derived substances regulating vasoconstriction and vessel growth. CAEC also modulate the expression of cellular adhesion molecules to control and fine-tune inflammatory responses and fibrinolysis. These physiological properties allow CAEC cultures to be widely used in the study of mechanisms for endothelium dysfunction, pathogenesis of coronary heart disease and atherosclerosis, and the development of novel disease treatments.
HCAEC from ScienCell Research Laboratories are isolated from human coronary artery. HCAEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HCAEC are characterized by immunofluorescence with antibodies specific to vWF/Factor VIII and/or CD31 (PECAM1). HCAEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HCAEC are guaranteed to further expand for 10 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Endothelial Cell Medium (ECM, Cat. #1001) for culturing HCAEC in vitro.
| Catalog No. | 6020 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HCAEC |
| Size/Quantity | 5 x 10⁵ cells/vial. |
| Product Use | HCAEC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures. |
| Storage | Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Elhadj, S. and Forsten, K. E. (2001) Chronic shear stress affects bovine aortic endothelial cell secreted proteoglycan production in vitro. Bioengineering Conference, Vol. 50:767-768. [2] Donnini, D., Perrella, G., Stel, G., Ambesi-Impiombato, F. S., Curcio, F. (2000) A new model of human aortic endothelial cells in vitro. Biochimie 82:1107-14. [3] Vassanelli C, Menegatti G, Zanolla L, Molinari J, Zanotto G, Zardini P. (1994) Coronary vasoconstriction in response to acetylcholine after balloon angioplasty: possible role of endothelial dysfunction. Coron Artery Dis. 5:979-986. [4] Meurice T, Vallet B, Bauters C, Dupuis B, Lablanche JM, Bertrand ME. (1996) Role of endothelial cells in restenosis after coronary angioplasty. Fundam Clin Pharmacol. 10:234-242. |
60201.) Sun, R.L., Huang, C.X., Bao, J.L., Jiang, J.Y., Zhang, B., Zhou, S.X., Cai, W.B., Wang, H., Wang, J.F. & Zhang, Y.L.(2016) 'CCChemokine Ligand 2 (CCL2) Suppresses High Density Lipoprotein (HDL) Internalization and Cholesterol Efflux via CCChemokine Receptor 2 (CCR2) Induction and p42/44 Mitogenactivated Protein Kinase (MAPK) Activation in Human Endothelial Cells *' J Biol Chem. VOL 291
2.) Shi, K.L., Qian, J.Y., Qi, L., Mao, D.B., Chen, Y., Zhu, Y. & Guo, X.G.(2016) 'Atorvastatin antagonizes the visfatininduced expression of inflammatory mediators via the upregulation of NFkB activation in HCAECs' Oncol Lett. VOL 12
3.) Guo T, Fang M, Zhang D, Li X. (2013) "Combination treatment with asiaticoside and rapamycin: A new hope for in?stent restenosis." ExpTher Med.6: 557
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| MEDIA | |||||
| 1001 | ECM | Endothelial Cell Medium |
$139.00
As low as:
$92.00
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| 8248 | BPF | Bovine Plasma Fibronectin |
$137.00
|
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| MOLECULAR BIOLOGY | |||||
| 6024 | HCAEC cDNA | Human Coronary Artery Endothelial Cell cDNA |
$453.00
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| 6025 | HCAEC tRNA | Human Coronary Artery Endothelial Cell Total RNA |
$401.00
|
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| 6026 | HCAEC Lysate | Human Coronary Artery Endothelial Cell Lysate |
$438.00
|
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| 6027 | HCAEC miRNA | Human Coronary Artery Endothelial Cell MicroRNA |
$374.00
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| 6029 | HCAEC gDNA | Human Coronary Artery Endothelial Cell Genomic DNA |
$438.00
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