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- Human Dermal Fibroblasts-adult
Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies as they are one of easiest types of cells to grow in culture. Their durability also makes them amenable to a variety of manipulations ranging from studies employing gene transfection to microinjection. In general, fibroblasts secrete a non-rigid extracellular matrix which is rich in type I and/or type III collagen. There is evidence showing that fibroblasts in various organs are intrinsically different. Dermal fibroblasts switch from a proliferative, migratory phase to a contractile, matrix-remodeling phase during wound healing. In addition, they secrete large quantities of hyaluronan in response to inflammatory stimuli.
HDF-a from ScienCell Research Laboratories are isolated from adult human skin. HDF-a are cryopreserved at passage one and delivered frozen. Each vial contains > 5 x 10^5 cells in 1 ml volume. HDF-a are characterized by their spindle morphology and immunofluorescence with antibody specific to fibronectin. HDF-a are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HDF-a are guaranteed to further expand for 15 population doublings under the
conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing HDF-a in vitro.
| Catalog No. | 2320 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HDF-a |
| Size/Quantity | 5 x 10^5 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Conrad, G. W., Hart, G. W., Chen, Y. (1977) Differences in vitro between fibroblast-like cells from cornea, heart, and skin of embryonic chicks. J. Cell Sci. 26:119-137. [2] Gabbiani, G., Rungger-Brandle, E., The fibroblast. In Tissue Repair and Regeneration (L. E. Glynn, ed.), pp 1-50. Handbook of Inflammation, Vol. 3. Amsterdam, Elsevier, 1981. [3] Stair S, Carlson KW, Shuster S, Wei ET, Stern R (2002) Mystixin peptides reduce hyaluronan deposition and edema formation. Eur J Pharmacol 30;450(3):291-6. |
23201.) Lee, K.I., Lee, S.Y. & Hwang, D.Y. (2016) Extracellular Matrix-Dependent Generation of Integration- and Xeno-Free iPS Cells Using a Modified mRNA Transfection Method Stem Cells Int. 2016
2.) Janmaat, C.J., de Rooij, K.E., Locher, H., de Groot, S.C., de Groot, J., Frijns, J.H.M. & Huisman, M.A. (2015) Human Dermal Fibroblasts Demonstrate Positive Immunostaining for Neuron- and Glia- Specific Proteins PLoS One. 10
3.) Rouabhia M, Park H, Meng S, Derbali H, Zhang Z. (2013) "Electrical Stimulation Promotes Wound Healing by Enhancing Dermal Fibroblast Activity and Promoting Myofibroblast Transdifferentiation." PLoS One. 8: e71660.
4.) Ploeger DT, Hosper NA, Schipper M, Koerts JA, de Rond S, Bank RA. (2013) "Cell plasticity in wound healing: paracrine factors of M1/ M2 polarized macrophages influence the phenotypical state of dermal fibroblasts." Cell Commun Signal. 11: 29. doi: 10.1186/1478-811X-11-29.
5.) Huang SM, Cheung CW, Chang CS, Tang CH, Liu JF, Lin YH, Chen JH, Ko SH, Wong KL, Lu DY. (2011) "Phloroglucinol derivative MCPP induces cell apoptosis in human colon cancer." J Cell Biochem. 112: 643-52.
6.) Kang TH, Guibinga GH, Jinnah HA, Friedmann T. (2011) "HPRT deficiency coordinately dysregulates canonical Wnt and presenilin-1 signaling: a neuro-developmental regulatory role for a housekeeping gene " PLoS One. 6: e16572.
7.) Lee W, Debasitis JC, Lee VK, Lee JH, Fischer K, Edminster K, Park JK, Yoo SS. (2009) " Multi layered culture of human skin fibroblasts and keratinocytes through three-dimensional freeform fabrication. " Biomaterials. 30: 1587-95.
8.) Hao S, Baltimore D. (2009) "The stability of mRNA influences the temporal order of the induction of genes encoding inflammatory molecules." Nat Immunol. 10: 281-8.
9.) Lee W, Debasitis JC, Lee VK, Lee JH, Fischer K, Edminster K, Park JK, Yoo SS. (2009) "Multi-layered culture of human skin fibroblasts and keratinocytes through three-dimensional freeform fabrication." Biomaterials. 30: 1587-95.
10.) Montes CL, Chapoval AI, Nelson J, Orhue V, Zhang X, Schulze DH, Strome SE, Gastman BR. (2008) "Tumor-induced senescent T cells with suppressor function: a potential form of tumor immune evasion." Cancer Res. 68: 870-9.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| 2324 | HDF-a cDNA | Human Dermal Fibroblast-adult cDNA |
$453.00
|
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| 2325 | HDF-a tRNA | Human Dermal Fibroblast-adult Total RNA |
$401.00
|
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| 2326 | HDF-a Lysate | Human Dermal Fibroblast-adult Lysate |
$438.00
|
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| 2327 | HDF-a miRNA | Human Dermal Fibroblast-adult MicroRNA |
$374.00
|
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| 2329 | HDF-a gDNA | Human Dermal Fibroblast-adult genomic DNA |
$438.00
|
