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How can we help you today?
Need help with product selection and ordering?
Email us at sales@sciencellonline.com
Need help with scientific inquiries, technical assistance, product applications, and use?
Email us at techsupport@sciencellonline.com
Need help with issues concerning this website?
Email us at webmaster@sciencellonline.com
Have any marketing related inquiries?
Email us at marketing@sciencellonline.com
Need help ordering from abroad?
As the largest cell provider, we are available worldwide.
Please visit our Distributors page to select the company nearest to your location.
We are located at 1610 Faraday Ave, Carlsbad, CA 92008
Call us at (877) 602-8549 or (760) 602-8549
Fax us at (760) 602-8575
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Fibroblasts are mesenchymal cells derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies as they are one of easiest types of cells to grow in culture. Their durability also makes them amenable to a variety of manipulations ranging from studies employing gene transfection to microinjection. In general, fibroblasts secrete a non-rigid extracellular matrix which is rich in type I and/or type III collagen. There is evidence showing that fibroblasts in various organs are intrinsically different. Dermal fibroblasts switch from a proliferative, migratory phase to a contractile, matrixremodeling phase during wound healing. In addition, they secrete large quantities of hyaluronan in response to inflammatory stimuli.
HDF-f-mt from ScienCell Research Laboratories are isolated from fetal human skin. HDF-f-mt are cryopreserved at passage one and delivered frozen. Each vial contains > 5 x 10^5 cells in 1 ml volume. HDF-f-mt are characterized by their spindle morphology and immunofluorescence with antibody specific to fibronectin. HDF-f-mt are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HDF-f-mt are guaranteed to further culture under the conditions provided by ScienCell Research Laboratories; however, HDF-f-mt are not recommended for expanding as mitomycin C treatment prevents further cell proliferation.
Recommended Medium
It is recommended to use Fibroblast Medium (FM, Cat. #2301) for culturing HDF-f-mt in vitro.
Catalog No. | 2350 |
---|---|
Country of Manufacture | United States |
Product Code | HDF-f-mt |
Size/Quantity | 1 x 10^5 cells/vial |
Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. |
Shipping Info | Dry ice. |
References | [1] Conrad, G. W., Hart, G. W., Chen, Y. (1977) Differences in vitro between fibroblast-like cells from cornea,
heart, and skin of embryonic chicks. J. Cell Sci. 26:119-137. [2] Gabbiani, G., Rungger-Brandle, E., The fibroblast. In Tissue Repair and Regeneration (L. E. Glynn, ed.), pp 1- 50. Handbook of Inflammation, Vol. 3. Amsterdam, Elsevier, 1981. [3] Stair S, Carlson KW, Shuster S, Wei ET, Stern R (2002) Mystixin peptides reduce hyaluronan deposition and edema formation. Eur J Pharmacol 30;450(3):291-6. |
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We are an expanding biotechnology company whose mission is the research and development of cell and cell-related products for experimental use. About Sciencell