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- Human Dermal Lymphatic Endothelial Cells
The lymphatic system is an essential part of the immune system. It serves distinct yet complementary functions with the blood vascular system in maintaining tissue homeostasis, including interstitial protein transport, tissue fluid balance, pressure equilibrium, and development of cellular immunity. Although lymphatic capillary endothelial cells have many properties in common with the endothelium of blood vessels, they possess distinct structural characteristics reflecting specific functions. Lymphatic capillaries firmly integrate within the interstitium, which is connected to the extracellular matrix by fine strands of elastic fibers. These capillaries have absent or poorly developed basement membranes and lack surrounding mural cells. Lymphatic endothelium typically contains numerous invaginations and cytoplasmic vesicles, as well as, characteristic overlapping intercellular junctions. Lymphatic endothelial cells are important modifiers of tumor microenvironment and are
implicated in metastasis.
HDLEC from ScienCell Research Laboratories are isolated from human skin. HDLEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HDLEC are characterized by immunofluorescence with antibodies specific to vWF, podoplanin and/or Lyve1. HDLEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HDLEC are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Endothelial Cell Medium (ECM, Cat. #1001) for culturing HDLEC in vitro.
| Catalog No. | 2010 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HDLEC |
| Size/Quantity | 5 x 10⁵ cells/vial. |
| Product Use | HDLEC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures. |
| Storage | Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Swartz, A. and Skobe, M. (2001) Lymphatic function, lymphangiogenesis, and cancer metastasis. Microsc. Res. Tech. 55:92-99. [2] Leak, L. V. (1970) Electron microscopic observations on lymphatic capillaries and the structural components of the connective tissue-lymph interface. Microvasc. Res. 2, 361-391 [3] Podgrabinska, S., Braun, P., Velasco, P., Kloos, B., Pepper, M., Jackson, D. and Skobe, M. (2002) Molecular characterization of lymphatic endothelial cells. PNAS 99:16069-16074. |
20101.) Hong, H., Jiang, L., Lin, Y., He, C., Zhu, G., Du, Q., Wang, X., She, F. & Chen, Y. (2016) TNF-alpha promotes lymphangiogenesis and lymphatic metastasis of gallbladder cancer through the ERK1/2/AP-1/VEGF-D pathway BMC Cancer. 16
2.) Hong, H., He, C., Zhu, S., Zhang, Y., Wang, X., She, F. & Chen, Y. (2016) CCR7 mediates the TNF-à-induced lymphatic metastasis of gallbladder cancer through the ?ERK1/2 - AP-1? and ?JNK - AP-1? pathways J Exp Clin Cancer Res. 35
3.) Han, L., Zhang, M., Wang, M., Jia, J., Zhao, M., Fan, Y. & Li, X. (2016) High Mobility Group Box-1 Promotes Inflammation-Induced Lymphangiogenesis via Toll-Like Receptor 4-Dependent Signalling Pathway PLoS One. 11
4.) Li, X.P., Jing, W., Sun, J.J., Liu, Z.Y., Zhang, J.T., Sun, W., Zhu, W. & Fan, Y.Z. (2015) A potential small-molecule synthetic antilymphangiogenic agent norcantharidin inhibits tumor growth and lymphangiogenesis of human colonic adenocarcinomas through blocking VEGF-A,-C,-D/VEGFR-2,-3 ?multi-points priming? mechanisms in vitro and in vivo BMC Cancer. 15
5.) Li, J., Zhou, J., Zhang, D., Song, Y., She, J. & Bai, C. (2015) Bone marrow-derived mesenchymal stem cells enhance autophagy via PI3K/AKT signalling to reduce the severity of ischaemia/reperfusion-induced lung injury J Cell Mol Med. 19
6.) Cai, X., Zhang, W., Chen, G., Li, R.F., Sun, Y.F. & Zhao, Y.F. (2015) Mesenchymal status of lymphatic endothelial cell: enlightening treatment of lymphatic malformation Int J Clin Exp Med. 8
7.) Bouta EM, McCarthy CW, Keim A, Wang HB, Gilbert RJ, Goldman J. (2011) "Biomaterial guides for lymphatic endothelial cell alignment and migration." Acta Biomater. 7: 1104-13.
8.) Yang S, Zhu X, Cai L, Cheng H, Zhao R, Wang H, Zhao H, Wang Z. (2010) "Role of tumor-associated lymphatic endothelial cells in metastasis: a study of epithelial ovarian tumor in vitro." Cancer Sci. 101: 679-85.
9.) Zhang D, Li B, Shi J, Zhao L, Zhang X, Wang C, Hou S, Qian W, Kou G, Wang H, Guo Y. (2010) "Suppression of tumor growth and metastasis by simultaneously blocking vascular endothelial growth factor (VEGF)-A and VEGF-C with a receptor-immunoglobulin fusion protein." Cancer Res. 70: 2495-503.
10.) Yoon CM, Hong BS, Moon HG, Lim S, Suh PG, Kim YK, Chae CB, Gho YS. (2008) "Sphingosine-1-phosphate promotes lymphangiogenesis by stimulating S1P1/Gi/PLC/Ca2+ signaling pathways." Blood. 112: 1129-38.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| MEDIA | |||||
| 1001 | ECM | Endothelial Cell Medium |
$139.00
As low as:
$92.00
|
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| 8248 | BPF | Bovine Plasma Fibronectin |
$137.00
|
||
| MOLECULAR BIOLOGY | |||||
| 2014 | HDLEC cDNA | Human Dermal Lymphatic Endothelial Cell cDNA |
$453.00
|
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| 2015 | HDLEC tRNA | Human Dermal Lymphatic Endothelial Cell Total RNA |
$401.00
|
||
| 2016 | HDLEC Lysate | Human Dermal Lymphatic Endothelial Cell Lysate |
$438.00
|
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| 2017 | HDLEC miRNA | Human Dermal Lymphatic Endothelial Cell MicroRNA |
$374.00
|
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| 2019 | HDLEC gDNA | Human Dermal Lymphatic Endothelial Cell Genomic DNA |
$438.00
|
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