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- Human Dermal Microvascular Endothelial Cells
Blood vessels of the skin are composed of a number of cellular and non-cellular elements. The predominant cell type in all skin blood vessels is endothelial cells. Endothelial cells form the interface between intravascular and extravascular compartments and serve as a selective barrier for the diffusion of cells and macromolecules between these two compartments. While endothelial cells lining blood vessels of the skin share features of endothelial cells elsewhere in the body, they also exhibit several skin specific characteristics. They actively participate in a variety of physiological processes including wound healing, control of hemostasis, temperature regulation, and modulation of inflammation/leukocyte trafficking. Via signaling pathways controlling cell proliferation, quiescence, apoptosis, and senescence, the endothelial lining of blood vessels shows remarkable phenotypic and functional heterogeneity, which in turn allows the cutaneous microvasculature to be in a dynamic balance between maintenance and remodeling.
HDMEC from ScienCell Research Laboratories are isolated from human skin. HDMEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HDMEC are characterized by immunofluorescence with antibodies specific to vWF/Factor VIII and/or CD31 (PECAM1). HDMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HDMEC are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Endothelial Cell Medium (ECM, Cat. #1001) for culturing HDMEC in vitro.
| Catalog No. | 2000 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HDMEC |
| Size/Quantity | 5 x 10⁵ cells/vial. |
| Product Use | HDMEC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures. |
| Storage | Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Swerlick, R. A and Lawley, T. J. (1993) Role of microvascular endothelial cells in inflammation. J. Invest. Dermatol. 100:111S. [2] Springer, T. (1994) Traffic signals for lymphocyte recirculation and leukocyte emigration. Cell 76:301. [3] Aird, W. C. (2003) Endothelial cell heterogeneity. Crit. Care Med. 31(4):S221-30. [4] Chang, E., Yang, J., Nagavarapu, U. and Herron, S. (2002) Aging and survival of cutaneous microvasculature. J. Invest. Dermatol. 118:752. |
20001.) Avril M, Tripathi AK, Brazier AJ, Andisi C, Janes JH, Soma VL, Sullivan DJ Jr, Bull PC, Stins MF, Smith JD. (2012) "A restricted subset of var genes mediates adherence of Plasmodium falciparum-infected erythrocytes to brain endothelial cells." Proc Natl Acad Sci USA. 109: E1782-90.
2.) Claessens A, Adams Y, Ghumra A, Lindergard G, Buchan CC, Andisi C, Bull PC, Mok S, Gupta AP, Wang CW, Turner L, Arman M, Raza A, Bozdech Z, Rowe JA. (2012) "A subset of group A-like var genes encodes the malaria parasite ligands for binding to human brain endothelial cells." Proc Natl Acad Sci USA. 109: E1772-81.
3.) Laranjeira MS, Fernandes MH, Monteiro FJ. (2012) "Reciprocal induction of human dermal microvascular endothelial cells and humanmesenchymal stem cells: time-dependent profile in a co-culture system." Cell Prolif. 45: 320-34.
4.) Alter A, de L s leuc L, Van Thuc N, Thai VH, Huong NT, Ba NN, Cardoso CC, Grant AV, Abel L, Moraes MO, Alca s A, Schurr E. (2010) "Genetic and functional analysis of common MRC1 exon 7 polymorphisms in leprosy susceptibility." Hum Genet. 127: 337-48.
5.) Atkinson BT, Jasuja R, Chen VM, Nandivada P, Furie B, Furie BC. (2010) "Laser-induced endothelial cell activation supports fibrin formation." Blood. 116: 4675-83.
6.) Hardcastle J, Kurozumi K, Dmitrieva N, Sayers MP, Ahmad S, Waterman P, Weissleder R, Chiocca EA, Kaur B. (2010) "Enhanced antitumor efficacy of vasculostatin (Vstat120) expressing oncolytic HSV-1." Mol Ther. 18: 285-94.
7.) Shahani T, Lavend'homme R, Luttun A, Saint-Remy JM, Peerlinck K, Jacquemin M. (2010) "Activation of human endothelial cells from specific vascular beds induces the release of a FVIII storage pool." Blood. 115: 4902-9.
8.) Song X, Luo Y. (2010) "The regulatory mechanism of Hsp90alpha secretion from endothelial cells and its role in angiogenesis during wound healing." Biochem Biophys Res Commun. 398: 111-7.
9.) Tsuji T, Aoshiba K, Nagai A. (2010) "Alveolar cell senescence exacerbates pulmonary inflammation in patients with chronic obstructive pulmonary disease." Respiration. 80: 59-70.
10.) May JM, Qu ZC, Qiao H. (2009) "Transfer of ascorbic acid across the vascular endothelium: mechanism and self-regulation." Am J Physiol Cell Physiol. 297: C169-78.
11.) Mikula I, Durocher S, Martasek P, Mutus B, Slama-Schwok A. (2009) "Isoform-specific differences in the nitrite reductase activity of nitric oxide synthases under hypoxia." Biochem J. 418: 673-82.
12.) Xueyong L, Shaozong C, Wangzhou L, Yuejun L, Xiaoxing L, Jing L, Yanli W, Jinqing L. (2008) "Differentiation of the pericyte in wound healing: The precursor, the process, and the role of the vascular endothelial cell." Wound Repair Regen. 16: 346-55.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| MEDIA | |||||
| 1001 | ECM | Endothelial Cell Medium |
$139.00
As low as:
$92.00
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| 8248 | BPF | Bovine Plasma Fibronectin |
$137.00
|
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| MOLECULAR BIOLOGY | |||||
| 2004 | HDMEC cDNA | Human Dermal Microvascular Endothelial Cell cDNA |
$453.00
|
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| 2005 | HDMEC tRNA | Human Dermal Microvascular Endothelial Cell Total RNA |
$401.00
|
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| 2006 | HDMEC Lysate | Human Dermal Microvascular Endothelial Cell Lysate |
$438.00
|
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| 2007 | HDMEC miRNA | Human Dermal Microvascular Endothelial Cell MicroRNA |
$374.00
|
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| 2009 | HDMEC gDNA | Human Dermal Microvascular Endothelial Cell genomic DNA |
$438.00
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