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- Human Epidermal Keratinocytes-neonatal
The epidermal layer of the skin provides an essential function as a protective barrier against insults from the external environment. The predominant cell type in the epidermis is keratinocytes and they are located in the stratified squamous epithelia. Keratinocytes are named after keratin, which is the most abundant protein in this cell. Progenitors of keratinocytes reside and divide in the basal layer of the epidermis. They then differentiate, migrate towards the surface of epidermis, and eventually withdraw from the cell cycle permanently. Keratinocyte proliferation, differentiation, and programmed cell death are complex and carefully choreographed processes. Apart from their protective functions, keratinocytes express adhesion molecules and cytokines, further suggesting an implication in skin innate immunity, tissue homeostasis, wound healing, cancer development, and skin-based gene-therapy.
HEK from ScienCell Research Laboratories are isolated from neonatal human skin. HEK are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HEK are characterized by immunofluorescence with antibodies specific to cytokeratin-18 and/or cytokeratin-19. HEK are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. HEK are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Keratinocyte Medium (KM, Cat. #2101) for culturing HEK in vitro.
| Catalog No. | 2100 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HEK |
| Size/Quantity | 5 x 10^5 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Eckert, R. L., Efimova, T., Dashti, S. R., Balasubramanian, S., Deucher, A., Crish, J. F., Sturniolo, M. and Bone, F. (2002) Keratinocyte survival, differentiation, and death: many roads lead to mitogen-activated protein kinase. J Investig Dermatol Symp Proc 7(1):36-40. [2] Song, P. I., Park, Y. M., Abraham, T., Harten, B., Zivony, A., Neparidze, N., Armstrong, C. A. and Ansel, J. C. (2002) Human keratinocytes express functional CD14 and toll-like receptor 4. J Invest Dermatol 119(2):424-32. [3] de Panfilis, G., Semenza, D., Lavazza, A., Mulder, A. A., Mommaas, A. M. and Pasolini, G. (2002) Keratinocytes constitutively express the CD95 ligand molecule on the plasma membrane: an in situ immunoelectron microscopy study on ultracryosections of normal human skin. Br J Dermatol. 147(1):7-12. |
21001.) Cheung PF, Wong CK, Ho AW, Hu S, Chen DP, Lam CW. (2010) "Activation of human eosinophils and epidermal keratinocytes by Th2 cytokine IL-31: implication for the immunopathogenesis of atopic dermatitis." Int Immunol. 22: 453-67.
2.) Pearse DB, Shimoda LA, Verin AD, Bogatcheva N, Moon C, Ronnett GV, Welsh LE, Becker PM. (2003) "Effect of cGMP on lung microvascular endothelial barrier dysfunction following hydrogen peroxide." Endothelium. 10: 309-17.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| 2110 | HEK-a | Human Epidermal Keratinocytes-adult |
$453.00
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| 2120 | HEK-f | Human Epidermal Keratinocytes-fetal |
$477.00
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| CP2100 | HEK-n Cell Pellet | Human Epidermal Keratinocyte-neonatal Cell Pellet |
$241.00
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| MEDIA | |||||
| 2101 | KM | Keratinocyte Medium |
$119.00
As low as:
$77.00
|
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| MOLECULAR BIOLOGY | |||||
| 2104 | HEK cDNA | Human Epidermal Keratinocyte cDNA |
$453.00
|
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| 2105 | HEK tRNA | Human Epidermal Keratinocyte-neonatal Total RNA |
$401.00
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| 2106 | HEK Lysate | Human Epidermal Keratinocyte Lysate |
$438.00
|
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| 2107 | HEK miRNA | Human Epidermal Keratinocyte MicroRNA |
$374.00
|
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| 2109 | HEK gDNA | Human Epidermal Keratinocyte genomic DNA |
$438.00
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