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The human esophagus is lined by a non-keratinizing, stratified squamous epithelium whose apical cell membranes and intercellular junctional complexes form a barrier against the influx of luminal content. The barrier helps to reduce exposure of surface cells to changes in osmolality, which occurs frequently within the esophageal lumen. Histologically, the esophageal epithelium consists of two zones, the basal and differentiated zones. Cellular proliferation is limited to the basal zone, and the cells are thought to migrate from this area towards the esophageal lumen. Migration is associated with the initiation of differentiation and the sequential expression of differentiation markers. Human esophageal epithelial cells (HEsEpiC) are an excellent in vitro model to study esophageal epithelium physiology and the mechanisms of esophageal carcinogenesis.
HEsEpiC from ScienCell Research Laboratories are isolated from the human esophagus. HEsEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HEsEpiC are characterized by immunofluorescence with antibody specific to cytokeratin-18 and/or cytokeratin-19. HEsEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HEsEpiC can be further cultured under the conditions provided by ScienCell Research Laboratories; however, HEsEpiC are not recommended for expanding or long-term cultures due to limited expansion capacity.
Recommended Medium
It is recommended to use Epithelial Cell Medium-2 (EpiCM-2, Cat. #4121) for culturing HEsEpiC in vitro.
| Catalog No. | 2720 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HEsEpiC |
| Size/Quantity | 5 x 10^5 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1]. Orlando, RC. (2000) Gastroesophageal Reflux Disease: Offensive Factors and Tissue Resistance. New York: Dekker. [2]. Orlando, G.S., Tobey, N.A., Wang, P., Abdulnour-Nakhoul, S. and Orlando, R.C. (2002) Regulatory volume decrease in human esophageal epithelial cells. Am J Physiol Gastrointest Liver Physiol 283: G932-G937. [3]. Jankowski, J., Hopwood, D., Dover, R. and Wormsley, K.G. (1993). Development and growth of normal, metaplastic and dysplastic oesophageal mucosa: biological markers of neoplasia. Eur. J. Gastroenterol. Hepatol. 5:235-246. |
27201.) Zhao, J., Li, X., Yang, Y., Zhu, D., Zhang, C., Liu, D., Wu, K. & Zhao, S.(2016) 'Effect of YAP1 silencing on esophageal cancer' Onco Targets Ther. VOL 9
2.) Wang, L., Xing, J., Cheng, R., Shao, Y., Li, P., Zhu, S. & Zhang, S. (2015) Abnormal Localization and Tumor Suppressor Function of Epithelial Tissue-Specific Transcription Factor ESE3 in Esophageal Squamous Cell Carcinoma PLoS One. 10
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| MEDIA | |||||
| 4121 | EpiCM-2 | Epithelial Cell Medium-2 |
$135.00
As low as:
$90.00
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| MOLECULAR BIOLOGY | |||||
| 2724 | HEsEpiC cDNA | Human Esophageal Epithelial Cell cDNA |
$453.00
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| 2725 | HEsEpiC tRNA | Human Esophageal Epithelial Cell Total RNA |
$401.00
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| 2726 | HEsEpiC Lysate | Human Esophageal Epithelial Cell Lysate |
$438.00
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| 2727 | HEsEpiC miRNA | Human Esophageal Epithelial Cell MicroRNA |
$374.00
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| 2729 | HEsEpiC gDNA | Human Esophageal Epithelial Cell Genomic DNA |
$438.00
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