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- Human Esophageal Microvascular Endothelial Cells
Endothelial cells lining the microvasculature are known to play a critical “gatekeeper” role in the inflammatory process through their ability to recruit circulating immune cells into tissues and foci of inflammation. Studies show that human esophageal microvascular endothelial cells (HEsMEC) exhibit a strong inflammatory immune response to LPS, cytokines, and pH exposure, thus playing a critical regulatory role in esophageal inflammation. When exposed to acidic pH, HEsMEC express certain proteins, such as VCAM-1, but not others, such as ICAM-1. This expression pattern could play a role in the cellular response triggered by gastroesophageal reflux disease. HEsMEC cultures have enabled scientists to observe leukocyte binding, upregulation of cell adhesion molecules, and other cellular responses to LPS, cytokines and pH exposure and has shown that HEsMEC are intrinsically different from lower gastrointestinal endothelial cells.
HEsMEC from ScienCell Research Laboratories are isolated from human esophageal tissue. HEsMEC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HEsMEC are characterized by immunofluorescence with antibody specific to vWF/Factor VIII and/or CD31 (PECAM1). HEsMEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HEsMEC are guaranteed to further expand for 10 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Endothelial Cell Medium (ECM, Cat. #1001) for culturing HEsMEC in vitro.
| Catalog No. | 2700 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HEsMEC |
| Size/Quantity | 5 x 10⁵ cells/vial. |
| Product Use | HEsMEC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures. |
| Storage | Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1], [3]. Rafiee, P., Ogawa, H., Heidemeann, J., Li, MS., Aslam, M., Lamirand, TH., Risher, PJ., Graewin, SJ., Dwinell, MB., Johnson, CP., Shaker, R., Binion, DG. (2003) Isolation and characterization of human esophageal microvascular endothelial cells: mechanisms of inflammatory activation. Am J Physiol Gastroinest Liver Physiol. 285(6): G1277-92. [2]. Rafiee, P., Theriot, ME,. Nelson, VM., Heidemann, J., Kanaa, Y., Horowitz, SA., Roqaczewski, A., Johnson, CP., Ali, I., Shaker, R., Binion, DG. (2006) Human esophageal microvascular endothelial cells respond to acidic pH stree by PI3K/AKT and p38 MAPK-regulated induction of Hsp70 and Hsp27. Am J Physiol Cell Physiol. 291(5): C931-45. |
27001.) He Y, Wang Y, Li P, Zhu S, Wang J, Zhang S. (2011) "Identification of GPX3 epigenetically silenced by CpG methylation in human esophageal squamous cell carcinoma." Dig Dis Sci. 56: 681-8.
2.) Ito T, Sato F, Kan T, Cheng Y, David S, Agarwal R, Paun BC, Jin Z, Olaru AV, Hamilton JP, Selaru FM, Yang J, Matsumura N, Shimizu K, Abraham JM, Shimada Y, Mori Y, Meltzer SJ. (2011) "Polo-like kinase 1 regulates cell proliferation and is targeted by miR-593* in esophageal cancer." Int J Cancer. 129: 2134-46.
3.) Tsuchiya S, Fujiwara T, Sato F, Shimada Y, Tanaka E, Sakai Y, Shimizu K, Tsujimoto G. (2011) "MicroRNA-210 regulates cancer cell proliferation through targeting fibroblast growth factor receptor-like 1 (FGFRL1)." J Biol Chem. 286: 420-8.
4.) Qazi A, Pal J, Maitah M, Fulciniti M, Pelluru D, Nanjappa P, Lee S, Batchu RB, Prasad M, Bryant CS, Rajput S, Gryaznov S, Beer DG, Weaver DW, Munshi NC, Goyal RK, Shammas MA. (2010) "Anticancer activity of a broccoli derivative, sulforaphane, in barrett adenocarcinoma: potential use in chemoprevention and as adjuvant in chemotherapy." Transl Oncol. 3: 389-99.
5.) Rafiee P, Nelson VM, Manley S, Wellner M, Floer M, Binion DG, Shaker R. (2009) "Effect of curcumin on acidic pH-induced expression of IL-6 and IL-8 in human esophageal epithelial cells (HET-1A): role of PKC, MAPKs, and NF-kappaB." Am J Physiol Gastrointest Liver Physiol. 296: G388-98.
6.) Alvarez H, Rojas PL, Yong KT, Ding H, Xu G, Prasad PN, Wang J, Canto M, Eshleman JR, Montgomery EA, Maitra A. (2008) "Mesothelin is a specific biomarker of invasive cancer in the Barrett-associated adenocarcinoma progression model: translational implications for diagnosis and therapy." Nanomedicine. 4: 295-301.
7.) Ito T, Shimada Y, Kan T, David S, Cheng Y, Mori Y, Agarwal R, Paun B, Jin Z, Olaru A, Hamilton JP, Yang J, Abraham JM, Meltzer SJ, Sato F. (2008) "Pituitary tumor-transforming 1 increases cell motility and promotes lymph node metastasis in esophageal squamous cell carcinoma." Cancer Res. 68: 3214-24.
8.) Shammas MA, Qazi A, Batchu RB, Bertheau RC, Wong JY, Rao MY, Prasad M, Chanda D, Ponnazhagan S, Anderson KC, Steffes CP, Munshi NC, De Vivo I, Beer DG, Gryaznov S, Weaver DW, Goyal RK. (2008) "Telomere maintenance in laser capture microdissection-purified Barrett's adenocarcinoma cells and effect of telomerase inhibition in vivo." Clin Cancer Res. 14: 4971-80.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| MEDIA | |||||
| 1001 | ECM | Endothelial Cell Medium |
$139.00
As low as:
$92.00
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| 8248 | BPF | Bovine Plasma Fibronectin |
$137.00
|
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| MOLECULAR BIOLOGY | |||||
| 2704 | HEsMEC cDNA | Human Esophageal Microvascular Endothelial Cell cDNA |
$453.00
|
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| 2705 | HEsMEC tRNA | Human Esophageal Microvascular Endothelial Cells Total RNA |
$401.00
|
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| 2706 | HEsMEC Lysate | Human Esophageal Microvascular Endothelial Cell Lysate |
$438.00
|
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| 2707 | HEsMEC miRNA | Human Esophageal Microvascular Endothelial Cell MicroRNA |
$374.00
|
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| 2709 | HEsMEC gDNA | Human Esophageal Microvascular Endothelial Cell Genomic DNA |
$438.00
|
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