Human G-CSF ELISA Kit

Granulocyte colony-stimulating factor (G-CSF) is a member of the CSF family of hormone-like glycoproteins that regulates hematopoietic cell proliferation and differentiation. In semi-solid agar culture, it almost exclusively stimulates the colony formation of granulocytes from committed precursor cells. G-CSF is also known as colony stimulating factor-3, and a single gene of it codes for a 177 or 180 amino acid mature protein with a molecular weight 19,600. Functionally, it stimulates the proliferation and differentiation of the progenitor cells for granulocytes. The effect of G-CSF on myeloid leukemias is unique among colony stimulating factors in driving the leukemic cells from a self-renewing malignant state to a mature differentiated phenotype with the concomitant loss of tumorigenicity. Additionally, it prevents cardiac remodeling after myocardial infarction by activating the Jak-Stat pathway in cardiomyocytes. The recombinant form of hG-CSF is capable of supporting neutrophil proliferation in a CFU-GM assay as well as early erythroid colonies and mixed colony formation. The human gene coding for G-CSF is assigned to the q21-q22 region of chromosome 17. The standard product used in this kit is recombinant human G-CSF, consisting of 175 amino acids with the molecular mass of 18.8KDa.

ScienCell's human G-CSF ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human G-CSF specific polyclonal antibody is pre-coated onto 8 x 12 divisible strips. The human G-CSF specific detection polyclonal antibody is biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates re washed away with TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human G-CSF captured in strips.