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- Human Gingival Fibroblasts
Fibroblasts are mesenchymal cells which perform many vital functions during development and in adulthood. They are responsible for much of the synthesis of extracellular matrix in connective tissue and play major roles in wound healing. Many diseases are associated with fibroblasts, either because fibroblasts are implicated in their etiology or because of the fibrosis that accompanies damage to other cell types. Fibroblasts are one of the most accessible mammalian cell types and one of the easiest types of cells to grow in culture. Gingival fibroblasts are the major constituents of gingival tissue and play a key role in their maintenance. Human gingival fibroblasts (HGnF) express a wide variety of surface molecules including CD9, CD26, CD55, CD59, CD63, CD71, CD86 CD95, CD99 and CD117. They also express mRNAs for protease-activated receptor-1 (PAR-1) and PAR-3.
HGnF from ScienCell Research Laboratories are isolated from human gingiva. HGnF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HGnF are characterized by their spindle morphology and immunofluorescence with antibodies specific to fibronectin. HGnF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HGnF are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium (FM, Cat. #2301) for the culturing of HGnF in vitro.
| Catalog No. | 2620 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HGnF |
| Size/Quantity | 5 x 10^5 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Poggi, P., Rodriguez y Baena, R., Rizzo, S., Rota, M. T. (2003) Mouthrinses with alcohol: cytotoxic effects on human gingival fibroblasts in vitro. J Periodontol. 74(5):623-9. [2] Di Domenico, G., Del Vecchio, L., Postiglione, L., Ramaglia, L. (2003) Immunophenotypic analysis of human gingival fibroblasts and its regulation by Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF). Minerva Stomatol. 52(3):81-7, 87-91. [3] Tanaka, N., Morita, T., Nezu, A., Tanimura, A., Mizoguchi, I., Tojyo, Y. (2003) Thrombin-induced Ca2+ mobilization in human gingival fibroblasts is mediated by protease-activated receptor-1 (PAR-1). Life Sci. 73(3):301-10. |
26201.) Herath TDK, Darveau RP, Seneviratne CJ, Wang C, Wang Y, Jin L. (2013) "Tetra- and penta-acylated lipid A structures of porphyromonas gingivalis LPS differentially activate TLR4-mediated NF-B signal transduction cascade and immuno-inflammatory response in human gingival fibroblasts." PLoS One. 8: e58496.
2.) Herath TDK, Wang Y, Seneviratne CJ, Darveau RP, Wang C, Jin L. (2013) "The expression and regulation of matrix metalloproteinase-3 is critically modulated by Porphyromonas gingivalis lipopolysaccharide with heterogeneous lipid A structures in human gingival fibroblasts." BMC Microbiol. 13: 73.
3.) Zhang K, Cheng L, Imazato S, Antonucci JM, Lin NJ, Lin-Gibson S, Bai Y, Xu HHK. (2013) "Effects of dual antibacterial agents MDPB and nano-silver in primer on microcosm biofilm, cytotoxicity and dentin bond properties." J Dent. 41: 464-74.
4.) Mitsak AG, Kemppainen JM, Harris MT, Hollister SJ. (2011) "Effect of polycaprolactone scaffold permeability on bone regeneration in vivo." Tissue Eng Part A. 17: 1831-9.
5.) Semlali A, Leung KP, Curt S, Rouabhia M. (2011) "Antimicrobial decapeptide KSL-W attenuates Candida albicans virulence by modulating its effects on Toll-like receptor, human beta-defensin, and cytokine expression by engineered human oral mucosa." Peptides. 32: 859-67.
6.) Kim DS, Kim JH, Lee GH, Kim HT, Lim JM, Chae SW, Chae HJ, Kim HR. (2010) "p38 Mitogen-activated protein kinase is involved in endoplasmic reticulum stress-induced cell death and autophagy in human gingival fibroblasts." Biol Pharm Bull. 33: 545-9.
7.) Kioi M, Shimamura T, Nakashima H, Hirota M, Tohnai I, Husain SR, Puri RK. (2009) "IL-13 cytotoxin has potent antitumor activity and synergizes with paclitaxel in a mouse model of oral squamous cell carcinoma." Int J Cancer. 124: 1440-8.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| 2624 | HGF cDNA | Human Gingival Fibroblast cDNA |
$453.00
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| 2625 | HGF tRNA | Human Gingival Fibroblast Total RNA |
$401.00
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| 2626 | HGnF Lysate | Human Gingival Fibroblast Lysate |
$438.00
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| 2627 | HGF miRNA | Human Gingival Fibroblast MicroRNA |
$374.00
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| 2629 | HGF gDNA | Human Gingival Fibroblast genomic DNA |
$438.00
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