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- Human Hepatic Sinusoidal Endothelial Cells
Endothelial cells perform multiple physiological functions and are central to many pathological processes. The liver contains two distinct endothelial cell types: vascular and sinusoidal. Sinusoidal endothelial cells (SEC) are microvascular endothelial cells with a unique phenotype reminiscent of dendritic cells and a unique function as antigen-presenting cells for CD4+ T cells. Thus, SEC represent a new type of organ-resident "non-professional" antigen-presenting cell that appears to be involved in the local control of the immune response and the induction of immune tolerance in the liver. The hepatic microenvironment, including the portal venous constituents and soluble mediators from sinusoidal cell populations, tightly control antigen presentation by SEC to avoid immune-mediated damage. SEC express well-characterized surface receptors and differ morphologically and metabolically from large-vessel endothelia. Previous studies have shown that SEC are dynamic regulators of porosity that respond rapidly and locally to environmental zonal stimuli during liver regeneration. SEC dysfunction and structural alterations have far-reaching repercussions for the whole liver.
HHSEC from ScienCell Research Laboratories are isolated from human liver. HHSEC are cryopreserved immediately after purification and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HHSEC are characterized by immunofluorescence with antibodies specific to vWF/Factor VIII and CD31 (PECAM). HHSEC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HHSEC are guaranteed to further expand for 5 population doublings under the conditions provided by ScienCell Research Laboratories. Note: HHSEC are not expected to proliferate many times in culture, so we recommend using cells for experiments at earliest passage after initial plating.
Recommended Medium
It is recommended to use Endothelial Cell Medium (ECM, Cat. #1001) for the culturing of HHSEC.
| Catalog No. | 5000 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HHSEC |
| Size/Quantity | 5 x 10⁵ cells/vial. |
| Product Use | HHSEC are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic procedures. |
| Storage | Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Limmer A, Knolle PA. (2001) Liver sinusoidal endothelial cells: a new type of organ-resident antigen-presenting cell. Arch Immunol Ther Exp (Warsz) 49 Suppl 1:S7-11. [2] Wack KE, Ross MA, Zegarra V, Sysko LR, Watkins SC, Stolz DB. (2001) Sinusoidal ultrastructure evaluated during the revascularization of regenerating rat liver. Hepatology 33(2):363-78. [3] Gervaz P, Scholl B, Mainguene C, Poitry S, Gillet M, Wexner S. (2000) Angiogenesis of liver metastases: role of sinusoidal endothelial cells. Dis Colon Rectum 43(7):980-6. |
50001.) Xie, F., Li, Z.P., Wang, H.W., Fei, X., Jiao, Z.Y., Tang, W.B., Tang, J. & Luo, Y.K. (2016) Evaluation of Liver Ischemia-Reperfusion Injury in Rabbits Using a Nanoscale Ultrasound Contrast Agent Targeting ICAM-1 PLoS One. 11
2.) Soronen, J., Yki?J„rvinen, H., Zhou, Y., S„devirta, S., Sarin, A., Leivonen, M., Sevastianova, K., Perttil„, J., Laurila, P., Sigruener, A., Schmitz, G. & Olkkonen, V.M. (2016) Novel hepatic microRNAs upregulated in human nonalcoholic fatty liver disease Physiol Rep. 4
3.) Koudelkova, P., Weber, G. & Mikulits, W. (2015) Liver Sinusoidal Endothelial Cells Escape Senescence by Loss of p19ARF PLoS One. 10
4.) Li, J., Zhou, J., Zhang, D., Song, Y., She, J. & Bai, C. (2015) Bone marrow-derived mesenchymal stem cells enhance autophagy via PI3K/AKT signalling to reduce the severity of ischaemia/reperfusion-induced lung injury J Cell Mol Med. 19
5.) Hu, C.T., Guo, L.L., Feng, N., Zhang, L., Zhou, N., Ma, L.L., Shen, L., Tong, G.H., Yan, Q.W., Zhu, S.J., Bian, X.W., Lai, M.D., Deng, Y.J. & Ding, Y.Q. (2015) MIF, secreted by human hepatic sinusoidal endothelial cells, promotes chemotaxis and outgrowth of colorectal cancer in liver prometastasis Oncotarget. 6
6.) Giugliano, S., Kriss, M., Golden-Mason, L., Dobrinskikh, E., Stone, A.E., Soto-Gutierrez, A., Mitchell, A., Khetani, S.R., Yamane, D., Stoddard, M., Li, H., Shaw, G.M., Edwards, M.G., Lemon, S.M., Gale, M., Shah, V.H. & Rosen, H.R. (2015) HCV Infection Induces Autocrine Interferon Signaling by Human Liver Endothelial Cell and Release of Exosomes, Which Inhibits Viral Replication Gastroenterology. 148
7.) Das A, Shergill U, Thakur L, Sinha S, Urrutia R, Mukhopadhyay D, Shah VH. (2013) "Ephrin B2/EphB4 pathway in hepatic stellate cells stimulates Erk-dependent VEGF production and sinusoidal endothelial cell recruitment." Am J Physiol Gastrointest Liver Physiol. 298: G908-15
8.) Lee SJ, Park SY, Jung MY, Bae SM, Kim IS. (2011) "Mechanism for phosphatidylserine-dependent erythrophagocytosis in mouse liver." Blood. 117: 5215-23.
9.) Das A, Shergill U, Thakur L, Sinha S, Urrutia R, Mukhopadhyay D, Shah VH. (2010) "Ephrin B2/EphB4 pathway in hepatic stellate cells stimulates Erk-dependent VEGF production and sinusoidal endothelial cell recruitment." Am J Physiol Gastrointest Liver Physiol. 298: G908-15.
10.) Guilini C, Urayama K, Turkeri G, Dedeoglu DB, Kurose H, Messaddeq N, Nebigil CG. (2010) "Divergent roles of prokineticin receptors in the endothelial cells: angiogenesis and fenestration." Am J Physiol Heart Circ Physiol. 298: H844-52.
11.) Huebert RC, Vasdev MM, Shergill U, Das A, Huang BQ, Charlton MR, LaRusso NF, Shah VH. (2010) "Aquaporin-1 facilitates angiogenic invasion in the pathological neovasculature that accompanies cirrhosis." Hepatology. 52: 238-48.
12.) Kim Y, Larkin AL, Davis RM, Rajagopalan P. (2010) "The design of in vitro liver sinusoid mimics using chitosan-hyaluronic acid polyelectrolyte multilayers." Tissue Eng Part A. 16: 2731-41.
13.) Tang L, Yang J, Liu W, Tang X, Chen J, Zhao D, Wang M, Xu F, Lu Y, Liu B, Sun Q, Zhang L, He F. (2009) "Liver sinusoidal endothelial cell lectin, LSECtin, negatively regulates hepatic T-cell immune response." Gastroenterology. 137: 1498-508.e1-5.
14.) Jung MY, Park SY, Kim IS. (2007) "Stabilin-2 is involved in lymphocyte adhesion to the hepatic sinusoidal endothelium via the interaction with alphaMbeta2 integrin." J Leukoc Biol. 82: 1156-65.
15.) Nahmias Y, Casali M, Barbe L, Berthiaume F, Yarmush ML. (2006) "Liver endothelial cells promote LDL-R expression and the uptake of HCV-like particles in primary rat and human hepatocytes." Hepatology. 43: 257-65.
16.) Nahmias Y, Odde DJ. (2006) "Micropatterning of living cells by laser-guided direct writing: application to fabrication of hepatic-endothelial sinusoid-like structures." Nat Protoc. 1: 2288-96.
17.) Perri RE, Langer DA, Chatterjee S, Gibbons SJ, Gadgil J, Cao S, Farrugia G, Shah VH. (2006) "Defects in cGMP-PKG pathway contribute to impaired NO-dependent responses in hepatic stellate cells upon activation." Am J Physiol Gastrointest Liver Physiol. 290: G535-42.
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| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| MEDIA | |||||
| 1001 | ECM | Endothelial Cell Medium |
$126.00
As low as:
$83.00
|
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| REAGENTS | |||||
| 0103 | T/E | Trypsin/EDTA Solution (0.25%; 100ml) |
$21.00
|
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| 0113 | TNS | Trypsin Neutralization Solution, 100 ml |
$21.00
|
||
| 0303 | DPBS | Dulbecco's Phosphate-Buffered Saline, 500 ml |
$16.00
|
||
| 0903 | EndoF | EndoFectagen |
$253.00
|
||
| 8248 | BPF | Bovine Plasma Fibronectin |
$124.00
|
||
| MOLECULAR BIOLOGY | |||||
| 5004 | HHSEC cDNA | Human Hepatic Sinusoidal Endothelial Cell cDNA |
$411.00
|
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| 5005 | HHSEC tRNA | Human Hepatic Sinusoidal Endothelial Cell Total RNA |
$364.00
|
||
| 5006 | HHSEC Lysate | Human Hepatic Sinusoidal Endothelial Cell Lysate |
$398.00
|
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| 5007 | HHSEC miRNA | Human Hepatic Sinusoidal Endothelial Cell MicroRNA |
$340.00
|
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| 5009 | HHSEC gDNA | Human Hepatic Sinusoidal Endothelial Cell genomic DNA |
$398.00
|
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