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Need help with product selection and ordering?
Email us at sales@sciencellonline.com
Need help with scientific inquiries, technical assistance, product applications, and use?
Email us at techsupport@sciencellonline.com
Need help with issues concerning this website?
Email us at webmaster@sciencellonline.com
Have any marketing related inquiries?
Email us at marketing@sciencellonline.com
Need help ordering from abroad?
As the largest cell provider, we are available worldwide.
Please visit our Distributors page to select the company nearest to your location.
We are located at 1610 Faraday Ave, Carlsbad, CA 92008
Call us at (877) 602-8549 or (760) 602-8549
Fax us at (760) 602-8575
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IGFBP1 is also known as amniotic fluid binding protein, placental protein-12, a-pregnancy-associated endometrial globulin, growth hormone independent binding protein, binding protein-28, binding protein-26, and binding protein-25.1 The low-molecular weight insulin-like growth-factor binding protein (IGF-BP25) is synthesized by the human liver, secretory endometrium and decidua, and it is also present in human serum. It binds insulin-like growth factors IGF-1 and IGF-2 with high affinity and is proposed to act as a paracrine regulator of cell growth.2 These IGF-binding proteins are expressed at different concentrations in different tissues and are thought to regulate the activity of IGF-1 and -2.3 The gene is organized in four exons and spans 5.9 kb.4 The IBP-1 gene is a single copy gene, located on chromosome 7.5The standard product used in this kit is recombinant human IGFBP-1, consisting of 234 amino acids with the molecular mass of 25KDa.
ScienCell's human IGFBP-1 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human IGFBP-1-specific polyclonal antibodies are pre-coated onto 8 x 12 divisible strips. The human specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies were subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human IGFBP-1 captured in strips.
Catalog No. | EK0382 |
---|---|
Country of Manufacture | China |
Product Code | hIGFBP-ELISA |
Size/Quantity | 96 Tests in 8 x 12 divisible strips |
Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
Storage | Store at 4°C For Frequent use, at -20°C For infrequent use. Avoid multiple Freeze-thaw cycles. |
Shipping Info | Gel ice. |
References | 1. Ballard, F. J.; Baxter, R. C.; Binoux, M.; Clemmons, D. R.; Drop, S. L. S.; Hall, K.; Hintz, R. L.; Rechler, M. M.;Rutanen, E. M.; Schwander, J. C. Report on the nomenclature of the IGF binding proteins. J. Clin. Endocr. Metab. 70: 817-818, 1990. 2. Alitalo, T.; Kontula, K.; Koistinen, R.; Aalto-Setala, K.; Julkunen, M.; Janne, O. A.; Seppala, M.; de la Chapelle, A. The gene encoding human low-molecular weight insulin-like growth-factor binding protein (IGF-BP25): regional localization to 7p12-p13 and description of a DNA polymorphism. Hum. Genet. 83: 335-338, 1989. 3. Ekstrand, J.; Ehrenborg, E.; Stern, I.; Stellan, B.; Zech, L.; Luthman, H. The gene for insulin-like growth factor binding protein-1 is localized to human chromosomal region 7p14-p1 2. Genomics 6: 413-418, 1990. 4. Brinkman, A.; Groffen, C. A. H.; Kortleve, D. J.; Drop, S. L. S. Organization of the gene encoding the insulin-like growth factor binding protein IBP-1. Biochem. Biophys. Res. Commun. 157: 898-907, 1988. 5. Brinkman, A.; Groffen, C.; Kortleve, D. J.; Geurts van Kessel, A.; Drop, S. L. S. Isolation and characterization of acDNA encoding the low molecular weight insulin-like growth factor binding protein (IBP-1). EMBO J. 7: 2417-2423,1988. |
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