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- Human Intestinal Smooth Muscle Cells
Smooth muscle contraction is the fundamental event in gastrointestinal motion. Although many of the biochemical mechanisms underlying the excitation-contraction coupling are not yet defined, it is known that cytosolic Ca2+ is the essential component in the coupling phenomenon. Inflammation of the human intestine causes increased levels of smooth muscle-specific actin, which in turn promotes the thickening of the smooth muscle layers. The increased smooth muscle actin may affect force production and further demonstrates the plasticity of smooth muscle cells in the inflamed intestine. Studies also show that human intestinal smooth muscle cells (HISMC) respond to IL-1beta and TNF-alpha stimulation by releasing IL-6, which may significantly contribute to the overall systemic inflammatory response. The availability of HISMC makes it more feasible to study the contractile and proliferative tissue responses of human intestinal smooth muscle.
HISMC from ScienCell Research Laboratories are isolated from human intestine. HISMC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HISMC are characterized by immunofluorescence with antibodies specific to α-smooth muscle actin. HISMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HISMC are guaranteed to further expand for 15 population doublings under the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Smooth Muscle Cell Medium (SMCM, Cat. #1101) for culturing HISMC in vitro.
| Catalog No. | 2910 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HISMC |
| Size/Quantity | 5 x 10⁵ cells/vial. |
| Product Use | HISMC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures. |
| Storage | Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1]. Blennerhassett, M. G., Bovell, F. M., Lourenssen, S., McHugh, K. M. (1999) Characteristics of inflammation-induced hypertrophy of rat intestinal smooth muscle cell. Dig Dis Sci. 44(7):1265-72. [2]. Ng, E. K., Panesar, N., Longo, W. E., Shapiro, M. J., Kaminski, D. L., Tolman, K. C., Mazuski, J. E. (2003) Human intestinal smooth muscle cells are potent producers of IL-6. Mediators Inflamm. 12(1):3-8. |
2910ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| MEDIA | |||||
| 1101 | SMCM | Smooth Muscle Cell Medium |
$126.00
As low as:
$86.00
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| MOLECULAR BIOLOGY | |||||
| 2914 | HISMC cDNA | Human Intestinal Smooth Muscle Cell cDNA |
$453.00
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| 2915 | HISMC tRNA | Human Intestinal Smooth Muscle Cell Total RNA |
$401.00
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| 2916 | HISMC Lysate | Human Intestinal Smooth Muscle Cell Lysate |
$438.00
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| 2917 | HISMC miRNA | Human Intestinal Smooth Muscle Cell MicroRNA |
$374.00
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| 2919 | HISMC gDNA | Human Intestinal Smooth Muscle Cell genomic DNA |
$438.00
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