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- Human Intrahepatic Biliary Epithelial Cells
Intrahepatic biliary epithelial cells (IBEpiC) line the intrahepatic biliary tree, a complex threedimensional network of tubular conduits of varying diameters within the liver. They comprise only 3 – 5% of the total population of liver cells, yet produce as much as 40% of the daily output of bile. A number of studies have demonstrated that IBEpiC play an important role in maintaining, modifying and augmenting the composition of canalicular bile by a coordinated series of hormoneregulated secretory and absorptive processes. IBEpiC also have an active immunologic role in both the innate and adaptive immune responses. IBEpiC secrete chemokines and cytokines and serve to localize the immune response by expressing critical cell adhesion molecules. Under basal conditions, IBEpiC are thought to be mitotically dormant, however, IBEpiC proliferate markedly in response to injury. Cultured Human IBEpiC (HIBEpiC) are a useful in vitro model for studying biliary cirrhosis and liver disease.
HIBEpiC from ScienCell Research Laboratories are isolated from human liver tissue. HIBEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HIBEpiC are characterized by immunofluorescence with antibody specific to cytokeratin18 and/or cytokeratin-19. HIBEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HIBEpiC are guaranteed to further expand for 10 population doublings in the condition provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Epithelial Cell Medium (EpiCM, Cat. #4101) for the culturing of HIBEpiC in vitro.
| Catalog No. | 5100 |
|---|---|
| Country of Manufacture | United States |
| Product Code | HIBEpiC |
| Size/Quantity | 5 x 10^5 cells/vial |
| Product Use | This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. |
| Storage | Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments. |
| Shipping Info | Dry ice. |
| References | [1] Roberts, S. K. and LaRusso, N. F. (1994) Pathobiology of biliary epithelia. Curr. Opin. Gastroenterol.
10:526-533. [2] Reynoso-Paz, S., Coppel, R. L., Mackay, I. R., Bass, N. M., Ansari, A. A. and Gershwin, M. E. (1999) The immunology of bile and biliary epithelium. Hepatology 30:351-357. [3] Lunz III, J. G., Contrucci, S., Ruppert, K., Nurase, N., Fung, J. J., Starzl, T. E. and Demetris, A. J. (2001) Replicative senescence of biliary epithelial cells precedes bile duct loss in chronic liver allograft rejection. Am. J. Pathol. 158:1379-1390. |
51001.) 2014 Oct;60(4):1314-23. doi: 10.1002/hep.27230. Epub 2014 Jul 31.
"Shotgun proteomics: identification of unique protein profiles of apoptotic bodies from biliary epithelial cells."
Lleo A1, Zhang W, McDonald WH, Seeley EH, Leung PS, Coppel RL, Ansari AA, Adams DH, Afford S, Invernizzi P, Gershwin ME.
2.) Gastroenterology. 2014 Jun;146(7):1795-808.e12. doi: 10.1053/j.gastro.2014.02.030. Epub 2014 Feb 25.
"Secretin stimulates biliary cell proliferation by regulating expression of microRNA 125b and microRNA let7a in mice."
Glaser S1, Meng F2, Han Y3, Onori P4, Chow BK5, Francis H2, Venter J3, McDaniel K6, Marzioni M7, Invernizzi P8, Ueno Y9, Lai JM10, Huang L10, Standeford H6, Alvaro D11, Gaudio E4, Franchitto A12, Alpini G13.
3.) Clin Exp Immunol. 2013 Apr;172(1):95-103. doi: 10.1111/cei.12046.
"Human intrahepatic biliary epithelial cells engulf blebs from their apoptotic peers."
Rong GH1, Yang GX, Ando Y, Zhang W, He XS, Leung PS, Coppel RL, Ansari AA, Zhong R, Gershwin ME.
4.) Biomed Res Int. 2013;2013:165715. doi: 10.1155/2013/165715. Epub 2013 Aug 20.
"LPS increases MUC5AC by TACE/TGF-α/EGFR pathway in human intrahepatic biliary epithelial cell."
Liu Z1, Tian F, Feng X, He Y, Jiang P, Li J, Guo F, Zhao X, Chang H, Wang S.
5.) PLoS One. 2013 Jul 26;8(7):e69496. doi: 10.1371/journal.pone.0069496. Print 2013.
"MiR-376c down-regulation accelerates EGF-dependent migration by targeting GRB2 in the HuCCT1 human intrahepatic cholangiocarcinoma cell line."
Iwaki J1, Kikuchi K, Mizuguchi Y, Kawahigashi Y, Yoshida H, Uchida E, Takizawa T.
6.) Am J Pathol. 2012 Sep;181(3):804-17. doi: 10.1016/j.ajpath.2012.06.010. Epub 2012 Jul 25.
"Epigenetic regulation of miR-34a expression in alcoholic liver injury."
Meng F1, Glaser SS, Francis H, Yang F, Han Y, Stokes A, Staloch D, McCarra J, Liu J, Venter J, Zhao H, Liu X, Francis T, Swendsen S, Liu CG, Tsukamoto H, Alpini G.
7.) Hepatology. 2012 Jan;55(1):209-21. doi: 10.1002/hep.24673.
"Role of stem cell factor and granulocyte colony-stimulating factor in remodeling during liver regeneration."
Meng F1, Francis H, Glaser S, Han Y, DeMorrow S, Stokes A, Staloch D, Venter J, White M, Ueno Y, Reid LM, Alpini G.
8.) Hepatology. 2011 Jul;54(1):196-203. doi: 10.1002/hep.24355.
"Epithelial cell specificity and apotope recognition by serum autoantibodies in primary biliary cirrhosis."
Rong G1, Zhong R, Lleo A, Leung PS, Bowlus CL, Yang GX, Yang CY, Coppel RL, Ansari AA, Cuebas DA, Worman HJ, Invernizzi P, Gores GJ, Norman G, He XS, Gershwin ME.
9.) Hepatology. 2009 Mar;49(3):871-9. doi: 10.1002/hep.22736.
"Apotopes and the biliary specificity of primary biliary cirrhosis."
Lleo A1, Selmi C, Invernizzi P, Podda M, Coppel RL, Mackay IR, Gores GJ, Ansari AA, Van de Water J, Gershwin ME.
ScienCell Research Laboratories (SRL) takes pride in being a resource for researchers all over the world. The publications listed here are not meant as an endorsement or confirmation of the reliability of the research methods. Our sole intention of sharing the research publications listed here is to provide research related insights and innovations of our products with other researchers.
| CAT. NO. | CODE | DESCRIPTION | PRICE | Qty | |
|---|---|---|---|---|---|
| MEDIA | |||||
| 4101 | EpiCM | Epithelial Cell Medium |
$135.00
As low as:
$90.00
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| MOLECULAR BIOLOGY | |||||
| 5004 | HHSEC cDNA | Human Hepatic Sinusoidal Endothelial Cell cDNA |
$453.00
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| 5005 | HHSEC tRNA | Human Hepatic Sinusoidal Endothelial Cell Total RNA |
$401.00
|
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| 5006 | HHSEC Lysate | Human Hepatic Sinusoidal Endothelial Cell Lysate |
$438.00
|
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| 5007 | HHSEC miRNA | Human Hepatic Sinusoidal Endothelial Cell MicroRNA |
$374.00
|
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| 5009 | HHSEC gDNA | Human Hepatic Sinusoidal Endothelial Cell genomic DNA |
$438.00
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